Rabbit anti-Human CD79b/Igβ Monoclonal Antibody for IF/ICC, FC - P40259

Core Product Specifications and Parameters

Biological Background: CD79b/Igβ Function and Localization

• CD79b (also known as Igβ, B29) is the beta chain of the B-cell antigen receptor complex, essential for B-cell development and function.
• Along with CD79a (Igα), CD79b initiates signal transduction upon BCR activation, leading to complex internalization, trafficking to late endosomes, and antigen presentation.
• CD79b enhances phosphorylation of CD79a, possibly by recruiting kinases or protecting CD79a from dephosphorylation.
• The protein contains an immunoglobulin domain and is classified as a member of the immunoglobulin superfamily.
• It is a transmembrane protein located on the cell membrane of B cells, where it forms disulfide-linked heterodimers with CD79a.
• CD79b undergoes post-translational modifications including phosphorylation and glycosylation, which are critical for its signaling function.
• The human CD79B gene maps to chromosome 17 and alternative splicing may generate different isoforms (as suggested by the UniProt keyword).
• Tissue expression is restricted to B cells, consistent with its role in adaptive immunity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 29-159), suggesting the antibody likely recognizes the native surface protein.
• For flow cytometry, consider using this antibody to detect surface CD79b on live B cells; validate with appropriate isotype controls.
• For immunofluorescence, optimize fixation and permeabilization conditions to preserve membrane localization and antigenicity.
• Avoid repeated freeze-thaw cycles of the antibody to maintain conjugate stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers investigating B-cell biology and immune signaling, we provide comprehensive sourcing solutions for rare targets like CD79b. From concept to clinic, CamelBio bridges your assay development needs with reliable, high-quality reagents.