Rabbit anti-Human CD79b/Igβ Monoclonal Antibody for IF/FC - P40259

Core Product Specifications and Parameters

Biological Background: CD79b/Igβ Function and Localization

• CD79b (also known as Igβ, B29, or CD79B) is the beta chain of the B-cell antigen receptor (BCR) complex, playing a critical role in B-cell signaling and adaptive immunity.
• It functions in cooperation with CD79a (Igα) to initiate the signal transduction cascade upon antigen binding, leading to BCR internalization, trafficking to late endosomes, and antigen presentation.
• CD79b enhances phosphorylation of CD79a, likely by recruiting Src-family kinases or protecting CD79a from dephosphorylation, thus amplifying the activation signal.
• The protein localizes to the cell membrane, specifically as a single-pass type I membrane protein, and is predominantly expressed in B lymphocytes.
• It contains an extracellular immunoglobulin-like domain, a transmembrane helix, and a cytoplasmic tail featuring ITAM motifs that are phosphorylated upon BCR engagement.
• Post-translational modifications include glycosylation, disulfide bond formation, and ubiquitination, which regulate its stability and signaling function.
• Alternative splicing produces multiple isoforms, and mutations in the CD79B gene are associated with certain B-cell immunodeficiencies and lymphomas.
• Categorized under adaptive immunity, CD79b is essential for B-cell development, proliferation, and differentiation.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated with , eliminating the need for a secondary antibody and reducing non-specific background in immunofluorescence and flow cytometry experiments.
• The immunogen corresponds to the extracellular domain (amino acids 29-159), making it ideal for detecting surface CD79b on live, non-permeabilized B cells.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific sample type, and include appropriate isotype controls.
• In immunofluorescence staining, consider using a nuclear counterstain (e.g., DAPI) to visualize cell nuclei and confirm membrane localization.
• Since CD79b is part of the BCR complex, co-staining with CD19 or CD20 can help identify B-cell populations; validate in your cell lines or primary samples.
• The predicted molecular weight is approximately 26 kDa for the full-length protein, but glycosylation may result in observed bands around 35-40 kDa in Western blotting; however, this antibody is not validated for WB, so use with caution or consider alternative antibodies for denaturing applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to validated IVD raw materials, including this -conjugated anti-CD79b antibody, which targets the B-cell receptor complex—an important marker in immunology and hematology research. With capabilities spanning monoclonal and polyclonal antibody development, bulk reagent supply, and rare target sourcing, CamelBio supports your project from concept to clinic.