Rabbit Anti-Human CD79B/Igβ Monoclonal Antibody () for Flow Cytometry - P40259

Core Product Specifications and Parameters

Biological Background: CD79B/Igβ Function and Localization

• CD79B, also known as B29, Ig-beta, or AGM6, is the beta chain of the B-cell antigen receptor complex. It is a type I transmembrane glycoprotein containing an extracellular immunoglobulin domain.
• In cooperation with CD79A (Ig-alpha), CD79B initiates the signal transduction cascade upon B-cell receptor (BCR) engagement, leading to receptor internalization and subsequent antigen presentation.
• CD79B enhances phosphorylation of CD79A, likely through the recruitment of kinases or by protecting CD79A from dephosphorylation, thereby amplifying BCR signaling.
• CD79B is predominantly expressed on the surface of B-cells, from early developmental stages through mature B-cells, and is essential for B-cell development and function.
• The protein localizes to the cell membrane as a component of the BCR complex, with its cytoplasmic tail containing immunoreceptor tyrosine-based activation motifs (ITAMs) for signal propagation.
• Post-translational modifications include phosphorylation on tyrosine residues within ITAMs, N-linked glycosylation, and disulfide bond formation, which are critical for its stability and signaling function.
• Mutations in the CD79B gene have been linked to primary immunodeficiencies and are observed in certain B-cell malignancies, highlighting its role in B-cell homeostasis.
• Alternative splicing of CD79B generates multiple isoforms, which may modulate BCR signaling dynamics.

Experimental Guidance and Technical Tips

• This antibody, raised against the extracellular region of CD79B, is ideal for detecting surface CD79B on live B cells by flow cytometry without the need for permeabilization, preserving cell viability.
• For flow cytometry assays, include proper compensation controls when using alongside other fluorochromes, as its emission profile may overlap with PE or similar dyes; consult spectral viewer tools for setup.
• Use an isotype control antibody conjugated to to assess non-specific binding and establish gating thresholds.
• Suitable sample sources include peripheral blood mononuclear cells (PBMCs), bone marrow samples, or dissociated lymphoid tissues. Co-staining with a B-cell marker (e.g., CD19) can confirm target specificity.
• Titration experiments are recommended to determine the optimal antibody concentration, reducing background and ensuring saturating binding for accurate quantification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic developers with high-quality reagents for immunology research. This anti-CD79B monoclonal antibody exemplifies our ability to supply validated monoclonal antibodies and bulk ancillary reagents for B-cell targeting applications. Whether you need established antibody pairs, custom antibody development, or rapid sourcing of rare targets, CamelBio streamlines your procurement from discovery to clinical manufacturing.