Rabbit anti-Human CD79b/Igβ Monoclonal Antibody for Flow Cytometry - P40259

Core Product Specifications and Parameters

Biological Background: CD79b/Igβ Function and Localization

• CD79b/Igβ is the beta chain of the B-cell antigen receptor (BCR) complex, essential for B-cell development and adaptive immunity.
• It cooperates with CD79A to initiate signal transduction upon antigen binding, triggering BCR internalization, trafficking to late endosomes, and subsequent antigen presentation.
• CD79b enhances phosphorylation of CD79A, likely by recruiting kinases or protecting CD79A from dephosphorylation, thus amplifying BCR signaling.
• The protein is specifically expressed in B-cells and localizes to the cell membrane, consistent with its role in surface antigen recognition.
• CD79b contains an immunoglobulin domain, is glycosylated and phosphorylated, and forms disulfide-linked heterodimers with CD79A for stable surface expression.
• Disease-associated variants in CD79B have been identified, linking this gene to B-cell-related immunodeficiencies and malignancies.
• Multiple isoforms exist due to alternative splicing, potentially contributing to functional diversity in BCR signaling.
• The mature protein has a calculated molecular weight of approximately 26 kDa, matching the ~26 kDa band detected by this antibody.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain of CD79b (amino acids 29–159), suggesting the antibody likely targets an extracellular epitope suitable for live-cell flow cytometry without permeabilization.
• For flow cytometry, titrate the antibody to determine the optimal staining concentration and include appropriate isotype and fluorescence-minus-one (FMO) controls.
• is a violet laser-excitable dye (405 nm excitation, 450 nm emission); verify that your flow cytometer is equipped with the correct laser and filter setup.
• To minimize nonspecific binding, block human Fc receptors and use viability dyes when staining primary B-cells or complex samples.
• Validate performance in your specific B-cell line or sample type, as expression levels may vary across maturation stages and pathological conditions.

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CamelBio provides diagnostic developers, laboratories, and research institutes with consolidated access to high-quality IVD raw materials, from validated monoclonal antibodies like this anti-CD79b clone to bulk ancillary reagents. Whether you need optimized antibody pairs for B-cell biomarker assays or assistance sourcing rare target materials, our one-stop platform supports your workflow from concept to clinic. Explore our catalog and technical services to accelerate your B-cell immunology and oncology projects.