Rabbit anti-Human CD79B/Igβ mAb for Flow Cytometry - P40259

Core Product Specifications and Parameters

Biological Background: CD79B/Igβ Function and Localization

• CD79B (Ig-beta, B29) is the beta chain of the B-cell antigen receptor complex, encoded by the CD79B gene.
• It cooperates with CD79A to initiate signal transduction upon BCR activation, leading to internalization, trafficking to late endosomes, and antigen presentation.
• CD79B enhances phosphorylation of CD79A, likely by recruiting kinases or protecting it from dephosphorylation.
• The protein is a single-pass type I membrane glycoprotein expressed exclusively on the surface of B-cells.
• It contains an extracellular immunoglobulin domain, a transmembrane helix, and a cytoplasmic tail with phosphorylation and ubiquitination sites.
• Post-translational modifications include glycosylation, disulfide bond formation, phosphorylation, and ubiquitination, which regulate signaling and trafficking.
• Variants in CD79B are associated with B-cell-related diseases, highlighting its critical role in adaptive immunity.

Experimental Guidance and Technical Tips

• This antibody is raised against a recombinant fusion protein covering the extracellular domain (aa 29–159), making it suitable for detecting surface CD79B.
• For flow cytometry, consider titrating the antibody and including appropriate isotype controls and Fc receptor blocking to reduce background.
• Validate antibody performance in your specific human B-cell sample system, as expression levels may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material sourcing partner, providing reliable access to antibodies like this anti-CD79B clone for B-cell research and immunodiagnostics. Beyond this product, we offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to streamline your assay development from concept to clinic.