Rabbit anti-Human CD75 Monoclonal Antibody for FC - P15907

Core Product Specifications and Parameters

Biological Background: CD75 (Beta-galactoside alpha-2,6-sialyltransferase 1) Function and Localization

• CD75, encoded by the ST6GAL1 gene, is a glycosyltransferase that transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates, playing a key role in glycosylation-dependent cellular processes.
• In B lymphocytes, CD75 generates neuraminidase-sensitive lymphocyte cell-surface differentiation antigens, such as CDw75, HB-6, and CD76, which are important for immune cell recognition and signaling. Related references: PMID:1730763
• CD75 sialylates complex-type N-glycans on the fragment crystallizable (Fc) region of IgGs, conferring anti-inflammatory effector functions and modulating antibody activity.
• The enzyme preferentially monosialylates the alpha(1->3) mannose antenna of Fc glycoforms, with subsequent disialylation occurring at a much slower rate, highlighting its substrate specificity.
• CD75 localizes primarily to the Golgi apparatus membrane, where it carries out its glycosyltransferase function, but it can also be secreted in soluble form under certain conditions.
• Alternative splicing of the ST6GAL1 gene generates isoforms with potentially distinct functions or localizations, as indicated by the presence of multiple transcript variants.
• Post-translational modifications, including disulfide bond formation, glycosylation, and phosphorylation, regulate CD75 stability and activity, reflecting its dynamic role in glycan synthesis.

Experimental Guidance and Technical Tips

• This -conjugated antibody is optimized for direct flow cytometry; titrate the antibody to determine the optimal concentration for your specific cell type and assay conditions.
• The immunogen corresponds to the extracellular region (amino acids 44-406), ensuring target accessibility on live cells; consider including appropriate isotype controls and Fc receptor blocking to minimize nonspecific binding.
• For human lymphocyte staining, validate the antibody in the relevant sample system (e.g., PBMCs or lymphoid cell lines) to confirm expected CD75 expression patterns.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with seamless access to high-quality IVD raw materials, including this CD75 monoclonal antibody for B-cell immunophenotyping. As a one-stop sourcing partner, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents—ensuring you have the critical tools for glycobiology and immuno-oncology workflows. Whether you need rare-target raw materials or comprehensive procurement support, CamelBio bridges your project from concept to clinic.