Rabbit anti-Human CD75 Monoclonal Antibody for FC - P15907

Core Product Specifications and Parameters

Biological Background: CD75 Function and Localization

• Protein identity: Beta-galactoside alpha-2,6-sialyltransferase 1 (ST6Gal I, ST6GAL1, SIAT1) is the enzyme responsible for generating the B-cell differentiation antigen CDw75 (PubMed:1730763). Related references: PMID:1730763
• Function: Catalyzes transfer of sialic acid from CMP-sialic acid to galactose-containing acceptors, sialylating complex-type N-glycans on IgG Fc and conferring anti-inflammatory properties.
• Lymphocyte antigen generation: Specifically generates neuraminidase-sensitive lymphocyte cell-surface differentiation antigens CDw75, HB-6 and CD76 in B cells.
• Localization: Predominantly a Golgi stack membrane protein; a proteolytically processed secreted form also exists.
• PTMs and isoforms: Subject to N-glycosylation, disulfide bond formation, and phosphorylation; alternative splicing yields multiple isoforms.
• Keywords: Glycosyltransferase, sialyltransferase, transmembrane helix, signal-anchor, proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen covers the luminal catalytic domain (aa 44–406), suggesting recognition of the ectodomain or secreted form; this may be advantageous for detecting surface-expressed CD75 in flow cytometry.
• Because the antibody is directly conjugated to , it is ready for use without a secondary reagent; titrate on B-lymphocyte lines to determine the optimal concentration for your assay.
• To confirm specificity, consider staining ST6GAL1 knockout or knockdown cells alongside wild-type controls.
• CD75 can be shed or secreted; using appropriate isotype controls and comparing permeabilized versus non-permeabilized staining can help distinguish surface from intracellular pools.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supporting sialyltransferase research and B-cell marker development. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw materials, helping you move seamlessly from concept to clinic.