Rabbit Anti-Human CD74 Monoclonal Antibody (PE) for Flow Cytometry - P04233-2

Core Product Specifications and Parameters

Biological Background: CD74 Function and Localization

• CD74 is the HLA class II histocompatibility antigen gamma chain, encoded by the CD74 (DHLAG) gene in humans.
• It is commonly referred to as the invariant chain (Ii) or HLA-DR antigens-associated invariant chain, and it associates with MHC class II molecules.
• This UniProt record describes isoform p35 (P04233-2) with a calculated molecular mass of approximately 26 kDa.
• Multiple CD74 isoforms likely exist, as indicated by the observed protein weights of 18, 24, 26, 31, and 34 kDa in different contexts.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal staining concentration and always include appropriate isotype controls.
• The immunogen covers amino acids 73–232, which lies within the extracellular domain; confirm that the antibody recognizes the specific CD74 isoform relevant to your experimental setup.
• PE is a bright fluorophore suitable for detecting low-abundance antigens; ensure proper compensation in multicolor panels.
• Validate antibody performance on known CD74-positive cells (e.g., B lymphocytes or professional antigen-presenting cells) before applying to unknown samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers streamlined access to validated IVD raw materials, including monoclonal antibodies, recombinant proteins, and ancillary reagents for immunology and MHC class II research. Our portfolio covers bulk-ready anti-CD74 clones, optimized antibody pairs, and rare-target sourcing to support your diagnostic development from concept to clinic. Partner with us to accelerate your assay workflow with reliable, one-stop supply solutions.