Rabbit anti-Human CD74 mAb - P04233-2

Core Product Specifications and Parameters

Biological Background: CD74 Function and Localization

• CD74 is a component of the HLA class II histocompatibility complex, acting as an invariant chain (Ii) that associates with MHC class II αβ heterodimers.
• Alternative names include HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, and CD74 antigen.
• The p35 isoform (UniProt P04233-2) has a calculated molecular weight of 26,399 Da and is one of several splice variants encoded by the CD74 gene.
• Alternative initiation and splicing generate multiple protein isoforms with molecular weights ranging from 18 kDa to 34 kDa, reflecting its complex regulatory roles.
• This protein is crucial for the intracellular trafficking of MHC class II molecules and the regulation of antigen presentation.

Experimental Guidance and Technical Tips

• The ABflo® 488 fluorophore allows direct detection by flow cytometry in the green channel (excitation 488 nm, emission 520 nm).
• Use appropriate isotype control antibodies and viable cell gating to ensure specificity.
• Optimal antibody dilution and staining protocols should be empirically determined for each experimental system.
• The immunogen spans residues 73–232, a region conserved across major human CD74 isoforms; this design likely enables broad isoform recognition.
• Validate antibody performance in the target cell type or tissue, as CD74 expression can vary with immune activation and cell lineage.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD developers, providing validated raw materials and technical support from concept to clinic. For CD74-related immunoassays and MHC class II research, we offer a range of matched antibody pairs, recombinant proteins, and ancillary reagents to accelerate diagnostic kit assembly. Leverage our supply capabilities for bulk monoclonal antibodies, optimized conjugates, and custom logistics to streamline your product development pipeline.