Rabbit anti-Human CD72 Monoclonal Antibody for Flow Cytometry - P21854

Core Product Specifications and Parameters

Biological Background: CD72 Function and Localization

• CD72 is a B-cell differentiation antigen (Lyb-2) encoded by the CD72 gene and is a single-pass type II membrane protein.
• Acts as a co-receptor of the B cell receptor (BCR), exerting both positive and negative regulatory effects on B-cell functions.
• Recognizes Sm/ribonucleoprotein self-antigen; coligation of CD72 with BCR inhibits BCR signaling through recruitment of PTPN6/SHP-1 to the BCR complex.
• Serves as a ligand for CD5, playing a critical role in maintaining regulatory T and B-cell homeostasis.
• Subcellular localization: Cell membrane; transmembrane protein.
• Tissue specificity: Expressed on pre-B-cells and B-cells but not on terminally differentiated plasma cells.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation (Keywords: Glycoprotein, Phosphoprotein, Disulfide bond).
• Predicted molecular weight is approximately 40 kDa (40220 Da).

Experimental Guidance and Technical Tips

• The immunogen covers extracellular domain amino acids 117-359, enabling recognition of native surface CD72; consider using this antibody for detection of cell surface CD72 by flow cytometry.
• For flow cytometry, titrate the antibody to determine the optimal working dilution for your specific cell type; use appropriate isotype controls and live/dead exclusion.
• This antibody is directly conjugated to , a far-red dye with excitation and emission suitable for multicolor panels; avoid combinations with overlapping fluorophores.
• Validate specificity on human B-cell lines (e.g., Raji, Daudi) or primary B cells; blocking with recombinant CD72 protein may serve as a negative control if background is observed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop platform providing diagnostic manufacturers, laboratories, and researchers with reliable IVD raw materials, technical services, and consulting—from concept to clinic. This anti-CD72 monoclonal antibody exemplifies our range of B-cell immunology tools, supporting studies on lymphocyte biology and autoimmune disease. Beyond standard antibodies, we offer validated antibody pairs, bulk ancillary reagents, and assistance in sourcing rare-target raw materials to streamline your procurement.