Rabbit anti-Human CD72 Monoclonal Antibody for FC - P21854

Core Product Specifications and Parameters

Biological Background: CD72 Function and Localization

• CD72, also known as Lyb-2, is a B-cell differentiation antigen and a type II transmembrane glycoprotein belonging to the C-type lectin superfamily.
• It functions as a co-receptor of the B cell receptor (BCR), playing both positive and negative regulatory roles in B-cell signaling.
• CD72 recognizes the Sm/ribonucleoprotein (RNP) self-antigen, and its coligation with BCR recruits PTPN6/SHP-1 to inhibit BCR signaling.
• It serves as a ligand for CD5, thereby contributing to the maintenance of regulatory T and B-cell homeostasis.
• CD72 is specifically expressed on pre-B-cells and mature B-cells, but not on terminally differentiated plasma cells.
• Subcellularly, CD72 is localized to the plasma membrane, consistent with its role as a transmembrane receptor.
• Post-translational modifications include disulfide bond formation, glycosylation, and phosphorylation, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , making it suitable for single-step flow cytometry staining. No secondary antibody is required.
• For optimal performance, titrate the antibody in the relevant sample system (e.g., human peripheral blood mononuclear cells or B-cell lines) to determine the ideal dilution for your assay.
• Include appropriate isotype controls and Fc receptor blocking to minimize nonspecific binding in flow cytometry experiments.
• Because the immunogen spans the extracellular domain (amino acids 117-359), the antibody likely recognizes an epitope accessible on live cells, making it suitable for surface staining.
• Validation in your specific application is recommended, as performance may vary with cell type, fixation/permeabilization protocols, and panel design.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to a comprehensive portfolio of IVD raw materials. This CD72 monoclonal antibody supports B-cell immunophenotyping studies, and we supply a full range of bulk ancillary reagents and rare target raw materials to power your assay development and production pipelines. From concept to clinic, rely on our integrated sourcing and consulting services to accelerate your diagnostic projects.