Rabbit anti-Human CD71 Monoclonal Antibody for Flow Cytometry - P02786

Core Product Specifications and Parameters

Biological Background: Transferrin Receptor Protein 1 (CD71) Function and Localization

• CD71 (TFRC) encodes transferrin receptor protein 1, a transmembrane glycoprotein that mediates cellular iron uptake through receptor-mediated endocytosis of iron-bound transferrin. Related references: PMID:26214738, PMID:41772062
• It plays a critical role in erythrocyte and nervous system development, and positively regulates T and B cell proliferation via iron acquisition. Related references: PMID:26642240
• CD71 functions as a lipid sensor; stearate levels modulate TFRC stearoylation, impacting JNK pathway activation and mitochondrial fusion through MFN2 degradation. Related references: PMID:26214738
• Subcellularly, CD71 is localized primarily to the cell membrane, and also found in melanosomes and secreted forms.
• It serves as a host cell entry receptor for several viruses, including arenaviruses, rabies virus, and coronaviruses (SARS-CoV, MERS-CoV, SARS-CoV-2), and is a receptor for Plasmodium vivax.
• Additional keywords include disulfide bond, glycoprotein, host-virus interaction, isopeptide bond, lipoprotein, palmitate, phosphoprotein, and Ubl conjugation.
• Alternative names for this target are T9, Transferrin receptor 1, p90, and TRFR.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100–250 within the extracellular domain, making this antibody well-suited for detecting surface CD71 in flow cytometry applications.
• For flow cytometry, titrate the antibody to determine optimal concentration for your specific cell type and fixation/permeabilization protocol.
• Consider using isotype controls and Fc receptor blocking to minimize non-specific binding.
• Validate performance in relevant human samples such as erythroid precursors, activated lymphocytes, or CD71-expressing cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated sourcing partner for IVD raw materials, offering a broad portfolio of validated antibodies, antigen pairs, and ancillary reagents to accelerate diagnostic development. This Rabbit anti-Human CD71 monoclonal antibody supports flow cytometric analysis, and its target is central to iron metabolism and viral entry research. Whether you need optimized clone pairs, bulk quantities, or rare target sourcing, CamelBio streamlines your path from concept to clinic.