Rabbit anti-Human CD70 Monoclonal Antibody for Flow Cytometry - P32970

Core Product Specifications and Parameters

Biological Background: CD70 Function and Localization

• CD70 (CD27 ligand, TNFSF7) is a type II transmembrane protein of the tumor necrosis factor (TNF) ligand superfamily, predominantly expressed on activated B cells and other antigen-presenting cells.
• Its receptor, CD27, is specifically expressed on T cells; the CD70-CD27 interaction provides co-stimulatory signals that drive antigen-specific T cell activation, proliferation, and survival, thereby linking innate and adaptive immunity. Related references: PMID:28011863 PMID:28011864 PMID:8387892
• The CD70-CD27 pathway is crucial for the formation and maintenance of T cell-dependent B cell responses, germinal center formation, and plasma cell differentiation.
• CD70 localizes to the cell membrane and contains a single transmembrane domain, an extracellular TNF homology domain responsible for receptor binding, and a cytoplasmic tail.
• Alternative splicing generates isoforms; the protein contains disulfide bonds and is N-glycosylated, which may contribute to its observed molecular weight of approximately 21–23 kDa.
• Aberrant or sustained CD70 expression has been implicated in autoimmune diseases and hematological malignancies, making it a therapeutic target of interest.

Experimental Guidance and Technical Tips

• This conjugate (Rabbit anti-Human CD70 mAb) is suitable for direct flow cytometric detection of CD70 on human B cells and other antigen-presenting cells; consider activation stimuli (e.g., CD40 ligation, TLR agonists) to upregulate CD70 expression in vitro.
• The immunogen corresponds to residues 39–193 of human CD70, encompassing the extracellular domain; epitope mapping may show specificity for the ligand-binding region.
• Perform antibody titration to identify the optimal staining concentration for your experimental system, and include appropriate isotype controls and Fc receptor blocking.
• While the product is validated for flow cytometry, other applications (e.g., immunohistochemistry, western blotting) may require testing under appropriate conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with reliable access to IVD-grade raw materials. In the area of immune checkpoint and TNF superfamily targets like CD70, we offer validated antibody pairs, optimized recombinant monoclonal antibodies, and ancillary reagents for immunoassay development. Whether you need bulk quantities or custom sourcing for rare targets, CamelBio serves as your bridge from concept to clinical application.