Rabbit anti-Human CD70 Monoclonal Antibody for Flow Cytometry - P32970

Core Product Specifications and Parameters

Biological Background: CD70 Function and Localization

• CD70 is expressed on the plasma membrane of B cells and functions as the ligand for the CD27 receptor specifically expressed on the surface of T cells. Related references: PMID:28011863 PMID:28011864 PMID:8387892
• The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion, providing immune surveillance of B cells. Related references: PMID:28011863 PMID:28011864
• CD70 is a single-pass type II transmembrane protein with a signal-anchor, localized at the cell membrane.
• It is a glycoprotein with disulfide bonds and undergoes alternative splicing.
• The unmodified molecular weight is approximately 21.1 kDa; glycosylation results in observed bands around 21–23 kDa, consistent with the provided protein weight.
• Mutations in CD70 are associated with disease variants, and it is a reference proteome target.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 39–193), suggesting the antibody may recognize native CD70 on live cells; consider this for non-permeabilized staining protocols.
• Validated for flow cytometry; for best results, titrate the antibody in your specific sample matrix and include appropriate isotype and viability controls.
• CD70 is expressed on activated B cells and other immune subsets; ensure your experimental system induces adequate expression if using stimulated cells.
• is excited by red lasers (e.g., 633 nm) and emits in the far-red; verify compatibility with your cytometer’s optical configuration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop sourcing platform for IVD raw materials, technical services, and consulting—from concept to clinic. This CD70 antibody supports immunology research, particularly for the CD27 pathway and T/B cell crosstalk. Beyond this product, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets to accelerate your assay development.