Rabbit anti-Human CD7 Monoclonal Antibody for Flow Cytometry - P09564

Core Product Specifications and Parameters

Biological Background: CD7 Function and Localization

• CD7 is a transmembrane glycoprotein belonging to the immunoglobulin superfamily, also known as GP40, LEU-9, Tp40, and TP41. It contains an immunoglobulin domain and is modified by disulfide bonds, glycosylation, and palmitoylation.
• CD7 functions as a costimulatory receptor on T-cells and NK cells, facilitating full activation upon binding to its ligand K12/SECTM1. Related references: PMID:10652336
• In T-cells, CD7 engagement promotes the production of interleukin-2 (IL-2), a critical growth factor for T-cell proliferation. Related references: PMID:1709867
• On resting NK cells, CD7 activation induces a significant increase in interferon-gamma (IFN-γ) secretion, enhancing innate immune responses. Related references: PMID:7506726
• CD7 is specifically expressed on the surface of T-lymphocytes and NK cells, as well as their precursors, consistent with its role in adaptive and innate immunity.
• The protein is localized to the plasma membrane, with a single-pass transmembrane helix and a cytoplasmic tail that contains palmitoylation sites, anchoring it to membrane lipid rafts.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal staining concentration for your cell population, and always include appropriate isotype controls.
• Since CD7 is expressed on T and NK cells, validate staining on peripheral blood mononuclear cells (PBMCs) or isolated lymphocyte subsets.
• Consider fixation and permeabilization protocols if intracellular staining is required, ensuring compatibility with the fluorophore.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for high-quality IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our CD7 antibody supports immunophenotyping research in T-cell and NK cell biology. We facilitate rare target raw-material sourcing and provide integrated technical and consulting services from concept to clinic.