Rabbit anti-Human CD7 Monoclonal Antibody for FC - P09564

Core Product Specifications and Parameters

Biological Background: CD7 Function and Localization

• CD7, also known as GP40, T-cell leukemia antigen, T-cell surface antigen Leu-9, and TP41, is a transmembrane glycoprotein encoded by the CD7 gene.
• It is expressed on T-cells and natural killer (NK) cells and their precursors (PubMed:7506726). Related references: PMID:7506726
• CD7 plays a costimulatory role in T-cell activation upon binding to its ligand K12/SECTM1 (PubMed:10652336). Related references: PMID:10652336
• It mediates the production of cytokines such as IL-2 (PubMed:1709867). Related references: PMID:1709867
• On resting NK cells, CD7 activation results in a significant induction of interferon-gamma levels (PubMed:7506726). Related references: PMID:7506726
• The protein is localized to the cell membrane and contains immunoglobulin domains, disulfide bonds, and palmitoylation sites.
• Its calculated molecular weight is approximately 25.4 kDa, consistent with the observed 25 kDa band.

Experimental Guidance and Technical Tips

• is a bright dye suitable for multicolor flow cytometry panels; consider titrating the antibody to determine the optimal staining concentration.
• Validate the antibody in the relevant sample system, such as human peripheral blood mononuclear cells (PBMCs) or T-cell lines, using standard FC protocols.
• Include appropriate isotype controls and Fc blocking steps to minimize nonspecific binding.
• The antibody targets an extracellular epitope (amino acids 26-180), so cell surface staining does not require permeabilization.

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