Rabbit anti-Human CD68 Monoclonal Antibody for Flow Cytometry (Intracellular) - P34810

Core Product Specifications and Parameters

Biological Background: Macrosialin (CD68) Function and Localization

• CD68, also known as macrosialin or GP110, is a heavily glycosylated transmembrane protein encoded by the CD68 gene on human chromosome 17. Alternative splicing produces multiple isoforms.
• It primarily functions in phagocytic activities of tissue macrophages, participating in intracellular lysosomal metabolism and extracellular cell-cell/cell-pathogen interactions.
• CD68 binds to tissue- and organ-specific lectins or selectins, facilitating macrophage homing to specific sites. Its rapid recycling from endosomes/lysosomes to the plasma membrane may support cellular crawling over selectin-bearing substrates.
• Highly expressed in blood monocytes and tissue macrophages, but also found in lymphocytes, fibroblasts, endothelial cells, and many tumor cell lines, where it may aid metastatic dissemination.
• Subcellularly, CD68 localizes to endosomes, lysosomes, and the cell membrane, consistent with its role in endocytic trafficking and surface adhesion.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, contributing to its stability and conformation.
• Keywords: Alternative splicing, Cell membrane, Disulfide bond, Endosome, Glycoprotein, Lysosome, Membrane, Signal, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to amino acids 22–319 of human CD68 (NP_001242.2), covering the luminal/extracellular domain, which may enable recognition of both membrane-bound and soluble forms.
• For intracellular flow cytometry (FC intra), consider permeabilization protocols to detect CD68 in endosomal/lysosomal compartments; validate optimal fixation and permeabilization conditions in your sample system.
• This antibody is reactive with human CD68. Cross-reactivity with other species has not been determined, so species specificity should be verified if used beyond human samples.
• As an direct conjugate, it eliminates the need for secondary antibody staining, simplifying multicolor panel design for macrophage and monocyte identification in complex cell mixtures.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop supplier for IVD raw materials, offering diagnostic manufacturers and research institutes streamlined access to high-quality antibodies, antigens, and ancillary reagents. This anti-CD68 monoclonal antibody supports macrophage-targeted research and potential diagnostic applications, including immune cell profiling and oncology biomarker studies. CamelBio’s portfolio also includes validated antibody pairs, bulk monoclonal/polyclonal antibodies, and custom sourcing for rare targets, enabling efficient scale-up from assay development to clinical manufacturing.