Rabbit anti-Human CD68 Monoclonal Antibody for Flow Cytometry (Intracellular) - P34810

Core Product Specifications and Parameters

Biological Background: Macrosialin (CD68) Function and Localization

• CD68 (macrosialin, Gp110) is a heavily glycosylated transmembrane protein belonging to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family.
• It plays a key role in phagocytic activities of tissue macrophages, participating in intracellular lysosomal metabolism and extracellular interactions with other cells and pathogens.
• CD68 binds to tissue- and organ-specific lectins or selectins, facilitating macrophage homing to particular sites.
• Rapid recycling of CD68 from endosomes and lysosomes to the plasma membrane may enable macrophage crawling over selectin-bearing substrates.
• Subcellularly, CD68 localizes primarily to endosomal and lysosomal membranes, but also cycles to the cell surface.
• It is highly expressed in blood monocytes and tissue macrophages, with lower expression in lymphocytes, fibroblasts, endothelial cells, and many tumor cell lines.
• Alternative splicing produces multiple isoforms, and the protein contains a signal peptide, a transmembrane helix, and luminal domains with disulfide bonds.
• Post-translational modifications include heavy N-linked glycosylation, contributing to its apparent molecular weight (~110 kDa; core protein ~37 kDa).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 22–319 of human CD68, covering a substantial extracellular/luminal portion. Consider its relevance for detecting both surface and intracellular pools after appropriate permeabilization.
• For intracellular flow cytometry (FC intra), use standard fixation and permeabilization protocols (e.g., with saponin or digitonin) to allow antibody access to endosomal/lysosomal compartments.
• Validate this antibody in your specific sample system (e.g., human PBMCs, tissue macrophages) to confirm staining patterns and optimal titers.
• Be aware that the conjugate may exhibit some background in heavily glycosylated or autofluorescent cells; include appropriate isotype controls and Fc receptor blocking.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams with streamlined access to validated IVD raw materials, including monoclonal antibodies like this CD68-specific clone. Whether you need bulk quantities of fluorescently labeled antibodies, matched antibody pairs, or support for rare-target reagent sourcing, we can help bridge your project from concept to clinic. For macrophage biology and immuno-oncology applications, explore our expanding catalog of conjugated primary antibodies and custom development services.