Rabbit anti-Human CD68 mAb - P34810

Core Product Specifications and Parameters

Biological Background: CD68 Function and Localization

• Macrosialin (CD68) is a heavily glycosylated transmembrane glycoprotein belonging to the LAMP family, encoded by the CD68 gene in humans.
• Plays a key role in phagocytic activities of tissue macrophages, including intracellular lysosomal metabolism and extracellular cell-cell/cell-pathogen interactions.
• Binds to tissue- and organ-specific lectins or selectins, facilitating homing of macrophage subsets to particular sites.
• Undergoes rapid recirculation from endosomes and lysosomes to the plasma membrane, potentially enabling macrophages to crawl over selectin-bearing substrates or other cells.
• Localizes to the cell membrane, endosome membrane, and lysosome membrane.
• Highly expressed in blood monocytes and tissue macrophages; also found in lymphocytes, fibroblasts, endothelial cells, and many tumor cell lines, which may aid in tumor cell adhesion and dissemination.
• Contains a signal peptide, a transmembrane helix, and multiple disulfide bonds; undergoes alternative splicing.
• Post-translational modifications include extensive N- and O-linked glycosylation.

Experimental Guidance and Technical Tips

• For flow cytometry (intracellular staining), permeabilize cells prior to staining to ensure antibody access to intracellular compartments where CD68 is abundant.
• Validate the antibody in your model system using known positive controls such as human macrophage cell lines (e.g., THP-1) or primary monocytes.
• Consider using a suitable isotype control and Fc receptor blocking to minimize non-specific binding.
• Optimize antibody concentration and staining conditions for your specific experimental setup, as CD68 expression levels can vary between cell types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for high-quality IVD raw materials, connecting diagnostic manufacturers and researchers with rigorously developed products like this anti-CD68 monoclonal antibody. With expertise in macrophage and tumor biology, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents in bulk, while also providing access to rare-target raw materials to accelerate your assay development from concept to clinic.