Rabbit anti-Human CD66b Monoclonal Antibody for Flow Cytometry - P31997

Core Product Specifications and Parameters

Biological Background: CD66b (CEACAM8) Function and Localization

• CD66b (CEACAM8) is a single-pass type I membrane protein and member of the carcinoembryonic antigen (CEA) family, also designated CD67, CGM6, and NCA-95.
• Functions as a calcium-independent cell adhesion molecule, mediating heterophilic interactions with other CEACAM family members such as CEACAM6 (PubMed:11590190, PubMed:2022629, PubMed:8776764). Related references: PMID:11590190, PMID:2022629, PMID:8776764
• Heterophilic adhesion between CEACAM8 molecules occurs specifically on activated neutrophils, contributing to immune cell interaction dynamics (PubMed:8776764). Related references: PMID:8776764
• Localized to the cell membrane and anchored via a glycosylphosphatidylinositol (GPI) moiety; contains immunoglobulin-like domains.
• Expressed predominantly in leukocytes of chronic myeloid leukemia patients and in bone marrow, consistent with a granulocytic lineage marker.
• The mature protein has a predicted molecular weight of ~38 kDa and undergoes N-linked glycosylation and disulfide bond formation.

Experimental Guidance and Technical Tips

• ABflo® 488 conjugate allows direct detection by flow cytometry; no secondary antibody is required. Optimize the antibody dilution (e.g., 0.5–5 µg per 10⁶ cells) using known positive and negative cell lines or primary human neutrophils.
• For surface staining, use live, unfixed cells to preserve epitope integrity; if fixation is necessary, validate that the chosen protocol does not alter CD66b recognition.
• Include appropriate isotype controls and fluorescence-minus-one (FMO) controls to assess background and set gates accurately.
• Not validated for other applications such as IHC, ICC, WB, or ELISA; users should conduct thorough pilot testing if adapting to these platforms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies procurement for diagnostic and research laboratories by offering integrated access to validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. For neutrophil biology and immune‑adhesion studies targeting CD66b and related CEACAM family members, our consolidated sourcing services help you secure rare‑target raw materials, technical consulting, and supply‑chain support from concept to clinic—all through one reliable partner.