Rabbit anti-Human CD66b Monoclonal Antibody for Flow Cytometry - P31997

Core Product Specifications and Parameters

Biological Background: CD66b (CEACAM8) Function and Localization

• CEACAM8 (CD66b) is a GPI-anchored cell surface glycoprotein belonging to the carcinoembryonic antigen (CEA) family.
• Functions as a calcium-independent cell adhesion molecule mediating heterophilic interactions with other CEACAMs, particularly CEACAM6, on activated neutrophils. Related references: PMID:11590190, PMID:2022629, PMID:8776764
• Plays a role in leukocyte adhesion and signaling during inflammatory responses.
• Expressed primarily on the surface of activated neutrophils and in leukocytes from chronic myeloid leukemia patients and bone marrow.
• Contains multiple immunoglobulin-like domains and disulfide bonds, contributing to its structural stability and binding properties.
• Predicted molecular weight of approximately 38 kDa, consistent with its 319-amino acid mature sequence.

Experimental Guidance and Technical Tips

• The immunogen covers a large extracellular region (aa 35–319), which includes the immunoglobulin-like domains responsible for ligand binding, making it suitable for detecting native cell surface CD66b in flow cytometry.
• For flow cytometry, consider using appropriate isotype controls and validate staining with known positive (e.g., activated human neutrophils) and negative cell populations.
• Titrate the antibody to optimize signal-to-noise ratio in your specific experimental system, and ensure proper sample preparation to maintain cell surface antigen integrity.
• Cross-reactivity with other family members should be assessed if multiplex staining is performed, though this antibody is reported to react specifically with human CD66b.

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CamelBio is your trusted one-stop partner for IVD raw material sourcing, providing comprehensive support from concept to clinic. Whether you need validated anti-CD66b antibodies for flow cytometric detection in leukocyte phenotyping, bulk ancillary reagents, or rare target procurement, our integrated supply chain ensures consistent quality and rapid delivery. Let us accelerate your diagnostic development in cell adhesion and hematology research.