Rabbit anti-Human CD66b Monoclonal Antibody for FC - P31997

Core Product Specifications and Parameters

Biological Background: CD66b Function and Localization

• CD66b (CEACAM8, CGM6, NCA-95, CD67) is a member of the carcinoembryonic antigen (CEA) gene family and the immunoglobulin superfamily, characterized by immunoglobulin-like domains and a C-terminal GPI anchor.
• It functions as a calcium-independent cell adhesion molecule, mediating heterophilic interactions with other CEACAM family members, particularly CEACAM6. Related references: PMID:11590190, PMID:2022629, PMID:8776764
• In activated neutrophils, CEACAM8 participates in cell-cell adhesion, potentially via homophilic or heterophilic binding, contributing to neutrophil functions. Related references: PMID:8776764
• The protein localizes to the cell membrane and cell surface, consistent with its role in extracellular adhesion.
• It is predominantly expressed in leukocytes from chronic myeloid leukemia patients and in bone marrow, indicating a myeloid lineage association.
• Post-translational modifications include N-glycosylation and disulfide bond formation; the protein contains a signal peptide and multiple immunoglobulin-like repeat domains.
• The predicted molecular weight is approximately 38 kDa (38154 Da), with a 38 kDa band commonly observed in experimental settings.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 35–319 of human CD66b, covering the extracellular immunoglobulin-like domains; this design is expected to target surface-exposed epitopes, making it well-suited for flow cytometry.
• As an -conjugated antibody, it is ready-to-use for direct immunofluorescence staining. Optimize the antibody dilution by titration to achieve the best signal-to-noise ratio in your specific experimental system.
• For positive controls, consider using human peripheral blood leukocytes or bone marrow samples, given the high expression of CD66b on neutrophils.
• Include appropriate isotype control antibodies to evaluate non-specific binding in FC experiments.
• Perform sample preparation and staining at 4°C with standardized protocols to minimize antigen modulation and ensure reproducibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This -conjugated anti-CD66b antibody supports neutrophil research and cell adhesion studies with reliable, ready-to-use materials. From concept to clinic, CamelBio offers comprehensive access to rare and routine raw materials, technical services, and consultation for diagnostic manufacturers, laboratories, and research institutes.