Rabbit anti-Human CD66a/CEACAM1 Monoclonal Antibody for FC - P13688

Core Product Specifications and Parameters

Biological Background: CEACAM1 Function and Localization

• CEACAM1 (also known as BGP-1, CD66a) is a cell adhesion molecule belonging to the carcinoembryonic antigen (CEA) family, encoded by the CEACAM1 gene (GeneID: 634) on chromosome 19.
• It functions as a calcium-independent homophilic cell adhesion molecule and a key coinhibitory receptor in immune responses, negatively regulating T cell receptor-mediated cytotoxicity, proliferation, and cytokine production, as well as NK cell cytolysis and neutrophil activation. Related references: PMID:18424730 PMID:23696226 PMID:25363763
• The protein is involved in insulin signaling by promoting insulin clearance and regulating lipogenesis, and it acts as an activator in angiogenesis, enhancing endothelial cell differentiation and vascular remodeling.
• Alternative splicing generates multiple isoforms; the predominant isoforms in T cells have a long cytoplasmic domain essential for signaling, while leukocytes primarily express isoforms 1 and 6.
• CEACAM1 localizes to various cell membrane domains (apical, basolateral, lateral), adherens junctions, microvilli, and secretory vesicle membranes; a secreted form is also produced.
• It is a highly glycosylated phosphoprotein with immunoglobulin-like domains and disulfide bonds; its phosphorylation is critical for recruiting phosphatases like PTPN6 (SHP-1) to mediate inhibitory signaling.
• Tissue expression is prominent in columnar epithelial cells of the colon, granulocytes, and lymphocytes; in T cells, expression is upregulated upon activation.
• CEACAM1 also plays roles in platelet aggregation inhibition, cell migration suppression via FLNA interaction, and bile acid transport. Related references: PMID:16291724

Experimental Guidance and Technical Tips

• The conjugate is optimized for flow cytometry; a starting titration of 0.5–5 µL per test (1×10^6 cells) is commonly used, but optimal dilution should be determined empirically.
• The immunogen (amino acids 35–428) corresponds to the extracellular domain, making this antibody suitable for surface staining of live, unfixed cells.
• Human peripheral blood mononuclear cells (PBMCs), T cell lines (e.g., Jurkat), or colorectal adenocarcinoma lines (e.g., HT-29) expressing CEACAM1 may be appropriate positive controls.
• Include an appropriate isotype control conjugated to the same fluorophore to assess nonspecific binding.
• Fc receptor blocking is recommended when staining cells with high FcγR expression (e.g., monocytes, macrophages) to reduce background.
• For multicolor panels, confirm minimal spectral overlap with using compensation controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw materials, offering a comprehensive range of antibodies, antigen pairs, and ancillary reagents to accelerate diagnostic development from concept to clinic. This anti-CEACAM1 antibody is an excellent tool for immunological and oncology research, and CamelBio can support your projects with bulk quantities, validated monoclonal/polyclonal pairs, and tailored sourcing of rare targets. Leverage our integrated services to streamline your procurement and ensure reliable supply for your immunoassay and diagnostic platforms.