Rabbit anti-Human CD66a/CEACAM1 Monoclonal Antibody for FC - P13688

Core Product Specifications and Parameters

Biological Background: CEACAM1 Function and Localization

• CEACAM1 (Biliary glycoprotein 1, BGP-1) is a member of the immunoglobulin superfamily that mediates calcium-independent homophilic cell adhesion, contributing to tissue organization and cell sorting. (By similarity)
• Acts as a coinhibitory receptor in immune cells: upon TCR stimulation, it recruits PTPN6 to dephosphorylate CD247 and ZAP70, inhibiting T-cell cytotoxicity and proliferation (PubMed:18424730). Related references: PMID:18424730
• In natural killer cells, CEACAM1 cis-interaction with KLRK1 leads to VAV1 dephosphorylation, suppressing NK-mediated cytolysis (PubMed:23696226). Related references: PMID:23696226
• Regulates T-cell responses via interaction with HAVCR2, playing a crucial role in autoimmunity and anti-tumor immunity (PubMed:25363763). Related references: PMID:25363763
• Modulates insulin signaling by promoting insulin clearance and regulating hepatic lipogenesis (By similarity).
• Functions as an activator in angiogenesis, enhancing endothelial cell migration and collateral artery growth after ischemia (By similarity).
• Inhibits cell migration and scattering by binding FLNA and disrupting FLNA-RALA interaction (PubMed:16291724). Related references: PMID:16291724
• Localized predominantly to the cell membrane (lateral, apical, basal), adherens junctions, and secreted forms; also detected in cytoplasmic vesicles.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal extracellular region (aa 35-428), encompassing multiple Ig-like domains; this design targets the native conformation and may detect both membrane-bound and soluble CEACAM1 forms.
• This conjugate is directly applicable for flow cytometry; titrate to determine optimal staining concentration for your cell type and experimental conditions.
• For best results, validate reactivity in your specific sample system, as CEACAM1 expression varies among cell types and activation states.
• Consider appropriate isotype controls and blocking steps to minimize nonspecific binding in FC assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing bridge for IVD raw materials, providing validated antibodies, bulk reagents, and rare target sourcing for research areas like immune checkpoint biology and cell adhesion. Whether you need ABflo® conjugated antibodies or custom development, CamelBio supports your diagnostic and research workflows from concept to clinic.