Rabbit anti-Human CD66a/CEACAM1 Monoclonal Antibody for FC - P13688

Core Product Specifications and Parameters

Biological Background: CEACAM1 Function and Localization

• CEACAM1 (Carcinoembryonic antigen-related cell adhesion molecule 1), also known as BGP-1, is a member of the carcinoembryonic antigen (CEA) immunoglobulin superfamily.
• It mediates calcium-independent homophilic cell adhesion, contributing to cell–cell interactions.
• CEACAM1 functions as a coinhibitory receptor in immune cells, suppressing TCR-mediated cytotoxicity and T cell proliferation by recruiting PTPN6 and dephosphorylating CD247 and ZAP70. Related references: PMID:18424730
• In natural killer (NK) cells, CEACAM1 inhibits KLRK1-mediated cytolysis through trans-homophilic binding and cis-interaction with KLRK1, leading to VAV1 dephosphorylation. Related references: PMID:23696226
• It negatively regulates neutrophil activity and IL1B production by facilitating a SYK-TLR4-CEACAM1 complex, and down-regulates neutrophil production via the CSF3R-STAT3 pathway.
• CEACAM1 also modulates insulin action by promoting INS clearance, regulates angiogenesis and vascular permeability, and inhibits cell migration through interaction with FLNA. Related references: PMID:16291724
• The protein is localized at the cell membrane (apical, basal, and lateral), cell junctions, adherens junctions, and can be secreted or found in cytoplasmic vesicles.
• It is expressed in colon epithelial cells, T cells, granulocytes, and lymphocytes, with leukocytes predominantly expressing isoforms containing a long cytoplasmic domain.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation, contributing to its structural and signaling roles.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal extracellular region (amino acids 35-428), ensuring reactivity against surface-exposed epitopes on live cells.
• This -conjugated antibody enables direct detection by flow cytometry without secondary reagents; consider using appropriate isotype controls and Fc receptor blocking steps.
• For optimal staining, titrate the antibody in your specific cell system and validate performance under your experimental conditions.
• Given its role in immune regulation, this antibody may be useful for studying CEACAM1 in T cells, NK cells, and tumor cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibodies and antigens for immune-oncology and cell adhesion research. For your CEACAM1/CD66a detection needs, we provide conjugated monoclonal antibodies, bulk reagents, and custom sourcing solutions. Partner with us to accelerate your assay development from target selection to clinical application.