Rabbit anti-Human CD62P/P-selectin Monoclonal Antibody for Flow Cytometry - P16109

Core Product Specifications and Parameters

Biological Background: P-selectin Function and Localization

• P-selectin (SELP) is a single-pass transmembrane glycoprotein belonging to the selectin family of adhesion molecules.
• It functions as a Ca2+-dependent lectin receptor that recognizes sialyl-Lewis X carbohydrates on myeloid cells, mediating tethering and rolling of leukocytes on activated endothelium or platelets.
• Through its interaction with P-selectin glycoprotein ligand-1 (SELPLG), it initiates leukocyte recruitment during inflammation.
• It also mediates cell-cell interactions and adhesion via integrins alpha-IIb/beta3 (ITGA2B:ITGB3) and integrin alpha-V/beta-3 (ITGAV:ITGB3) (PubMed:37184585). Related references: PMID:37184585
• Subcellularly, P-selectin is stored in secretory granules—α-granules of platelets and Weibel-Palade bodies of endothelial cells—and rapidly translocates to the cell surface upon cellular activation by agonists such as thrombin or histamine.
• The extracellular region comprises a lectin domain, an EGF-like domain, multiple sushi (CCP) repeats, and is modified by N-linked glycosylation and palmitoylation.
• Primarily expressed in platelets and endothelial cells, its surface expression serves as a marker of platelet and endothelial activation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 42-771) of P-selectin, which includes the lectin and EGF domains, likely providing high specificity for native P-selectin on activated cells.
• For flow cytometry experiments, consider using human whole blood or isolated platelets stimulated with agonists such as thrombin or histamine to upregulate surface P-selectin expression.
• Validate antibody performance in the relevant sample system, including appropriate isotype controls and compensation settings for the conjugate.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers with a comprehensive portfolio of IVD raw materials, including this monoclonal antibody for detection of P-selectin in inflammation and thrombosis research. Our supply capabilities encompass optimized antibody pairs, conjugated antibodies, and bulk ancillary reagents, alongside rare target raw-material sourcing to streamline assay development from concept to clinic.