Rabbit anti-Human CD62L/L-Selectin Monoclonal Antibody for Flow Cytometry - P14151

Core Product Specifications and Parameters

Biological Background: L-Selectin Function and Localization

• L-Selectin, encoded by the SELL gene, is also known as CD62L, LAM-1, Leu-8, LECAM1, and lymph node homing receptor, and belongs to the selectin family of cell adhesion molecules.
• It mediates calcium-dependent cell adhesion by binding to glycoprotein ligands on neighboring cells, promoting the initial tethering and rolling of leukocytes on endothelial surfaces – a critical step in lymphocyte homing to peripheral lymph nodes. Related references: PMID:12403782; PMID:28011641; PMID:28489325
• L-Selectin is a type I transmembrane glycoprotein located on the cell membrane, featuring an extracellular region with a C-type lectin domain, an EGF-like domain, and short consensus repeat (Sushi) domains.
• The protein is expressed on the surface of most peripheral blood B cells and T lymphocytes, as well as on subsets of natural killer cells, monocytes, and granulocytes.
• Alternative initiation of translation results in two isoforms with predicted molecular weights of approximately 42 kDa and 43 kDa, consistent with the observed doublet in some western blot analyses.
• Key post-translational modifications include N-linked glycosylation and disulfide bond formation, which are essential for proper folding and ligand-binding activity.
• L-Selectin shedding from the cell surface by proteolytic cleavage regulates leukocyte rolling velocity and transmigration.

Experimental Guidance and Technical Tips

• This antibody is a directly conjugated reagent; optimize staining concentration by titration in the experimental system.
• For flow cytometry, use fresh or properly cryopreserved human lymphocyte or leukocyte samples to maintain surface L-Selectin expression.
• Include appropriate isotype controls and Fc receptor blocking steps to minimize nonspecific binding.
• Note that L-Selectin is rapidly shed upon cell activation; consider staining under chilled conditions and in the presence of protease inhibitors if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to validated IVD raw materials, including this anti-human CD62L/L-Selectin monoclonal antibody for flow cytometry. Our portfolio covers optimized antibody pairs, recombinant proteins, and ancillary reagents for immunology and cell adhesion research. Contact us for bulk supply, custom conjugation, or assistance in sourcing rare-target raw materials to accelerate your diagnostic development.