Rabbit anti-Human CD62E/E-Selectin Monoclonal Antibody for Flow Cytometry - P16581

Core Product Specifications and Parameters

Biological Background: E-Selectin Function and Localization

• E-selectin, also known as CD62E, ELAM-1, or LECAM2, is a cell-surface glycoprotein encoded by the SELE gene. It belongs to the selectin family of adhesion molecules, characterized by an N-terminal lectin domain, an EGF-like domain, and multiple sushi (complement control protein) repeats.
• The primary function of E-selectin is to mediate leukocyte adhesion to activated endothelium during inflammation. It recognizes and binds to specific carbohydrate ligands, notably sialyl-Lewis X, on neutrophils, monocytes, and memory T cells.
• E-selectin is not constitutively expressed; its transcription is induced by cytokines such as tumor necrosis factor-alpha (TNF-α) and interleukin-1 (IL-1). This tightly regulated expression ensures a rapid and transient adhesion response at sites of injury or infection.
• Beyond its role in immune surveillance, E-selectin has been implicated in capillary morphogenesis, suggesting a function in angiogenesis and vascular remodeling.
• The protein is a type I transmembrane glycoprotein, anchored in the plasma membrane with its ligand-binding domains exposed on the cell surface. Its extracellular region contains multiple domains stabilized by disulfide bonds and calcium-binding sites.
• Key structural features include an N-terminal C-type lectin domain responsible for calcium-dependent carbohydrate recognition, followed by a single epidermal growth factor (EGF)-like domain and six short consensus repeats (sushi domains). The short cytoplasmic tail may interact with the cytoskeleton and intracellular signaling molecules.
• E-selectin undergoes post-translational modifications, including N-glycosylation and the formation of intrachain disulfide bonds, which are critical for its proper folding and ligand-binding activity.

Experimental Guidance and Technical Tips

• This antibody was developed using an immunogen corresponding to the extracellular region (amino acids 22-230) of human E-selectin, which encompasses the lectin and EGF-like domains. This design favors epitopes accessible in native conformations, making the clone well suited for flow cytometry applications.
• For flow cytometry staining, use a single-cell suspension prepared from activated endothelial cells or leukocyte populations. Consider titrating the antibody to determine the optimal signal-to-noise ratio for your specific model system.
• Include appropriate isotype controls and live/dead discrimination to ensure specificity. E-selectin expression may be low on resting cells, so cytokine stimulation (e.g., TNF-α treatment) is recommended to upregulate target expression.
• The conjugate is compatible with common red/orange laser lines (e.g., 561 nm or 594 nm) and can be combined with other fluorophores in multicolor panels. Validate spectral overlap compensation settings in your instrument.
• Always protect conjugated antibodies from light and avoid repeated freeze-thaw cycles to maintain fluorophore stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research laboratories, and institutes with one-stop access to high-quality IVD raw materials. This anti-E-selectin monoclonal antibody exemplifies our ability to deliver validated antibody reagents for cell adhesion and inflammation research. Beyond individual antibodies, we offer matched antibody pairs, bulk reagent supply, and customized sourcing for even the rarest targets, streamlining your path from concept to clinic.