Rabbit anti-Human CD61 Monoclonal Antibody for Flow Cytometry - P05106

Core Product Specifications and Parameters

Biological Background: Integrin beta-3 Function and Localization

• Integrin beta-3 (ITGB3, GPIIIa) is the beta subunit of two major heterodimeric integrins: alpha-IIb/beta-3 (platelet glycoprotein GPIIb/IIIa) and alpha-V/beta-3 (vitronectin receptor).
• The alpha-IIb/beta-3 integrin mediates platelet aggregation by binding soluble fibrinogen, leading to platelet-platelet interaction and hemostatic plug formation. Related references: PMID:9111081
• Integrin alpha-V/beta-3 (and alpha-IIb/beta-3) recognizes the R-G-D motif in a wide array of ligands including vitronectin, fibronectin, thrombospondin, and osteopontin, and serves as a receptor for fibrillin-1 (FBN1) and bone sialoprotein (IBSP), facilitating cell adhesion and migration. Related references: PMID:12807887, PMID:10640428
• ITGAV:ITGB3 acts as a coreceptor or binding partner for various growth factors and cytokines, including FGF1, FGF2, IGF1, IGF2, NRG1, IL1B, and CX3CL1, essential for their signaling pathways. Related references: PMID:18441324, PMID:28302677, PMID:19578119, PMID:28873464, PMID:20682778, PMID:29030430, PMID:23125415, PMID:24789099
• In the brain, ITGAV:ITGB3 participates in synaptic transmission and plasticity, regulating serotonin reuptake by localizing SLC6A4 and modulating AMPA receptor endocytosis, affecting synaptic strength.
• Integrin beta-3 is localized at the cell membrane, lamellipodium membrane, focal adhesions, and postsynaptic membrane, reflecting its roles in cell adhesion, migration, and synaptic functions.
• ITGB3 serves as a receptor for several viruses, including Herpes virus 8, Coxsackievirus A9, Hantaan virus, Cytomegalovirus, Human metapneumovirus, Human parechovirus 1, West Nile virus, and HIV-1 (via Tat protein), implicating it in viral entry and infection.
• Alternative splicing generates isoforms beta-3A (widely expressed, notably in osteoblasts) and beta-3C (prostate and testis), with a molecular weight of approximately 87 kDa.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine optimal concentration for your cell type and include appropriate isotype controls and viability dyes.
• The immunogen spans the majority of the extracellular domain (aa 21-718), suggesting recognition of native conformations; confirm specificity using CD61-positive cells such as platelets or endothelial cell lines.
• To reduce non-specific binding in FC, consider using Fc receptor blocking reagents and gentle cell harvesting techniques.
• This antibody may detect both beta-3A and beta-3C isoforms; validate in relevant tissue samples if isoform discrimination is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with high-quality IVD raw materials, including validated antibody pairs, monoclonal antibodies, and ancillary reagents. For integrin beta-3 (CD61) studies, we offer comprehensive sourcing solutions from concept to clinic, supporting platelet biology, adhesion research, and viral entry assays. Let CamelBio streamline your procurement with one-stop access to rare targets, bulk reagents, and technical consultation.