Rabbit anti-Human CD6 Monoclonal Antibody for Flow Cytometry (FC) - P30203

Core Product Specifications and Parameters

Biological Background: CD6 Function and Localization

• CD6 (T-cell differentiation antigen CD6), also known as T12 or TP120, is a cell adhesion molecule critical for T-cell regulation and immune responses. It mediates cell-cell contacts through interaction with ALCAM/CD166.
  Related references: PMID:15048703, PMID:15294938, PMID:16352806
• By engaging ALCAM, CD6 contributes to the formation and stabilization of the immunological synapse between T-cells and antigen-presenting cells.
  Related references: PMID:15294938, PMID:16352806
• CD6 serves as a costimulatory molecule, enhancing T-cell activation and proliferation via signaling cascades, including those downstream of the TCR/CD3 complex.
  Related references: PMID:15294938, PMID:16352806, PMID:16914752, PMID:24584089
• It also functions in innate immunity as a pattern recognition receptor for bacterial LPS and lipoteichoic acid, triggering MAP kinase activation and pro-inflammatory cytokine release.
  Related references: PMID:17601777
• CD6 localizes primarily to the cell membrane but can be secreted; it is expressed on thymocytes, peripheral T-cells, NK cells, and a small subset of B-cells, with soluble CD6 detectable in serum.
  Related references: PMID:15294938, PMID:15048703, PMID:16352806, PMID:17601777, PMID:9013954
• Multiple isoforms arise from alternative splicing, yielding proteins with molecular weights ranging from 58 to 71 kDa (UniProt canonical sequence 72 kDa).
• Post-translational modifications include N-glycosylation and phosphorylation, influencing CD6 localization and function.

Experimental Guidance and Technical Tips

• This -conjugated antibody is ready-to-use for flow cytometry, eliminating the need for a secondary antibody and reducing non-specific binding.
• Given the multiple CD6 isoforms, the antibody’s recombinant immunogen (aa 18-364) targets the extracellular domain; users should validate detection of all relevant isoforms in their specific cell system.
• Include appropriate isotype controls and Fc receptor blocking to minimize background staining, particularly when analyzing primary human immune cells.
• Titrate the antibody to determine the optimal concentration for your cell type and experimental condition; a typical starting dilution is 1:50–1:200 for 10^6 cells.
• Fixation and permeabilization may be used for intracellular staining, but test compatibility with the fluorophore conjugate beforehand.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio is a trusted partner for IVD raw material sourcing, offering a comprehensive portfolio of validated antibodies, antigens, and ancillary reagents to support diagnostic assay development from concept to clinic.
• This Rabbit anti-Human CD6 mAb is part of our immune cell marker collection, designed for researchers and manufacturers focusing on T-cell biology, immune modulation, and sepsis biomarker studies.
• We also supply matching antibody pairs, bulk quantities, and custom reagent services to streamline your workflow and reduce supply chain complexity.