Rabbit anti-Human CD59 Monoclonal Antibody for Flow Cytometry - P13987

Core Product Specifications and Parameters

Biological Background: CD59 Function and Localization

• CD59, also known as protectin, MAC-inhibitory protein (MAC-IP), membrane inhibitor of reactive lysis (MIRL), and HRF-20, is a GPI-anchored glycoprotein expressed on human cells.
• It functions as a potent inhibitor of the complement membrane attack complex (MAC), preventing damage to host cells by blocking the incorporation of multiple C9 molecules into the pore (PubMed:11882685, PubMed:1698710, PubMed:2475111, PubMed:36797260).
• Related references: PMID:11882685 PMID:1698710 PMID:2475111 PMID:36797260
• A soluble form of CD59 is found in urine, which retains complement binding activity but has significantly reduced capacity to inhibit MAC assembly on cell membranes.
• CD59 is localized on the cell membrane via a glycosylphosphatidylinositol (GPI) anchor; it is also secreted.
• Mutations in the CD59 gene are linked to hereditary hemolytic anemia, underscoring its essential role in protecting erythrocytes from complement-mediated lysis.
• The protein undergoes alternative splicing and post-translational modifications including disulfide bond formation, glycation, and GPI anchor addition.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 26-101 of human CD59, a region critical for its interaction with complement components C8 and C9. Consider using intact, live cells for surface staining due to the extracellular GPI anchor.
• For flow cytometry, optimal antibody concentrations should be empirically determined. A suggested starting dilution range is 1:50–1:200. Include appropriate isotype controls and compensation controls when using multiple fluorophores.
• This antibody is conjugated to , which is excited by the 405 nm violet laser and detected with a 450/50 nm bandpass filter. Ensure your cytometer configuration is compatible.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and healthcare institutions with comprehensive access to IVD raw materials, technical services, and supply chain solutions. For complement system research and CD59-targeted assay development, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare-target raw materials. Let us support your journey from concept to clinic with reliable, research-use antibodies and customizable procurement options.