Rabbit anti-Human CD59 Monoclonal Antibody for Flow Cytometry - P13987

Core Product Specifications and Parameters

Biological Background: CD59 Function and Localization

• CD59 glycoprotein, also known as protectin, MAC-inhibitory protein (MAC-IP), membrane inhibitor of reactive lysis (MIRL), or HRF-20, is a key regulator of the complement system.
• CD59 is a potent inhibitor of the complement membrane attack complex (MAC) and protects human cells from complement-mediated lysis. It binds to the beta-hairpins of C8 (C8A and C8B) in the assembling MAC, forming an intermolecular beta-sheet that prevents incorporation of multiple copies of C9 required for complete formation of the osmolytic pore. Related references: PMID:11882685, PMID:1698710, PMID:2475111, PMID:36797260
• The soluble form of CD59, found in urine, retains specific complement binding activity but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
• CD59 is primarily localized to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor but can also be secreted.
• Post-translational modifications include N-glycosylation, disulfide bond formation, and glycation, contributing to its structural stability and function.
• Mutations in CD59 are associated with hereditary hemolytic anemia.
• Alternative splicing of the CD59 gene generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region of CD59 (aa 26–101), which is exposed on the cell surface; the conjugate is suitable for direct flow cytometry detection of native CD59 without a secondary antibody.
• For flow cytometry, titrate the antibody to determine the optimal staining concentration for your specific cell type; excess antibody may increase background.
• Include appropriate isotype controls and viability dyes to distinguish specific membrane staining from non-specific binding to dead cells.
• CD59 expression can vary among cell types and activation states; validate the staining in the relevant biological system (e.g., peripheral blood mononuclear cells, tumor cell lines) and consider co-staining with other complement regulatory markers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of IVD raw materials, offering this anti-CD59 antibody as part of a comprehensive portfolio for complement research and immuno-oncology applications. For diagnostic manufacturers, CamelBio provides validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized rare target raw-material sourcing to support assay development. Our integrated supply chain and technical support help accelerate your projects from concept to clinic.