Rabbit anti-Human CD59 Monoclonal Antibody for FC - P13987

Core Product Specifications and Parameters

Biological Background: CD59 Function and Localization

• CD59 is a potent inhibitor of the complement membrane attack complex (MAC), thereby protecting human cells from complement-mediated damage during immune responses.
  Related references:
  PMID:11882685
  PMID:1698710
  PMID:2475111

• It exerts its inhibitory function by binding to the beta-hairpin structures of the C8 components (C8A and C8B) of the assembling MAC, forming an intermolecular beta-sheet that blocks the incorporation of multiple C9 molecules required for complete pore formation.
  Related references:
  PMID:11882685
  PMID:1698710
  PMID:36797260

• A soluble form of CD59, present in urine, retains complement-binding activity but exhibits a greatly reduced capacity to inhibit MAC assembly on cell membranes.

• CD59 is localized at the cell membrane via a glycosylphosphatidylinositol (GPI) anchor and is also secreted into the extracellular environment.

• The protein is glycosylated, contains disulfide bonds, and is known by multiple alternative names such as protectin, MAC-inhibitory protein (MACIP), and membrane inhibitor of reactive lysis (MIRL).

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fragment covering amino acids 26–101 of human CD59. It may recognize both the membrane-bound and soluble forms of the protein; verify specificity in your assay system.

• For flow cytometry (FC), consider using standard staining protocols with appropriate titration of the antibody to determine optimal concentration for your cell type of interest.

• CD59 is widely expressed on many human cell types, including erythrocytes and endothelial cells; include positive and negative controls to validate staining.

• Since CD59 is a GPI-anchored protein, ensure that sample preparation (e.g., cell fixation and permeabilization) is compatible with detection of surface-exposed epitopes.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw material supplier, CamelBio connects diagnostic manufacturers and researchers with high-quality reagents. This CD59 antibody supports complement and immunology research, and CamelBio offers one-stop access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents—streamlining your procurement from concept to clinic.