Rabbit anti-Human CD58 Monoclonal Antibody for FC - P19256

Core Product Specifications and Parameters

Biological Background: CD58 Function and Localization

• CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a cell surface glycoprotein that belongs to the immunoglobulin superfamily.
• It serves as the ligand for CD2, a T-lymphocyte glycoprotein, mediating cell adhesion between T cells and various target cells.
• The CD2-CD58 interaction is crucial for thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent T-cell interactions, and T-lymphocyte rosetting with erythrocytes.
• It plays a role in priming responses by both CD2+ and LFA-3+ cells, contributing to immune synapse formation and T-cell activation.
• CD58 is localized to the cell membrane and is a single-pass type I membrane protein.
• The protein undergoes alternative splicing, and the mature form contains disulfide bonds and glycosylation sites, characteristic of cell adhesion molecules.
• Keywords include: 3D-structure, Alternative splicing, Cell membrane, Direct protein sequencing, Disulfide bond, Glycoprotein, Immunoglobulin domain, Membrane, Proteomics identification, Reference proteome, Signal, Transmembrane, Transmembrane helix.
• Molecular weight of the unprocessed precursor is approximately 28 kDa.

Experimental Guidance and Technical Tips

• The immunogen was designed from a recombinant protein encompassing the extracellular domain (amino acids 29-215) of human CD58. This region is expected to generate antibodies that recognize the native conformation on live cells, making this antibody suitable for flow cytometry.
• For flow cytometry, consider titrating the antibody to determine the optimal concentration for your specific cell type. Include appropriate isotype controls and Fc receptor blocking to minimize nonspecific binding.
• Since CD58 is ubiquitously expressed on many hematopoietic and non-hematopoietic cells, ensure proper gating and compensation when performing multicolor flow cytometry.
• This antibody is validated for human samples only; cross-reactivity with other species is not expected based on the immunogen sequence homology.
• For functional studies, note that binding of this antibody to CD58 may block the CD2-CD58 interaction; thus, it could be explored as a blocking reagent in adhesion or activation assays if further validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents for IVD manufacturing. For researchers studying immune cell adhesion and T-cell activation, CamelBio ensures reliable access to high-quality anti-CD58 antibodies and can assist in sourcing rare-target raw materials for diagnostic development. As your one-stop partner from concept to clinic, we streamline the supply chain for your assay needs.