Rabbit anti-Human CD58 mAb for FC - P19256

Core Product Specifications and Parameters

Biological Background: CD58 Function and Localization

• CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a cell surface glycoprotein that serves as a ligand for the T-lymphocyte CD2 glycoprotein.
• The interaction between CD58 and CD2 mediates thymocyte interactions with thymic epithelial cells, facilitating T-cell development.
• CD58-CD2 binding is critical for both antigen-independent and antigen-dependent interactions of T-lymphocytes with target cells and antigen-presenting cells, playing a key role in immune response.
• CD58 is also involved in T-lymphocyte rosetting with erythrocytes, a characteristic feature of human T cells.
• Subcellularly, CD58 is located on the cell membrane and is a transmembrane protein with an immunoglobulin-like domain.
• The protein undergoes alternative splicing and contains disulfide bonds, and is post-translationally modified by glycosylation.
• CD58 has a molecular weight of approximately 28 kDa and is a member of the immunoglobulin superfamily.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 29–215), making this antibody suitable for detecting membrane-bound CD58 in flow cytometry.
• For flow cytometry, titrate the antibody to determine the optimal working dilution for your specific experimental setup.
• Include appropriate isotype controls and viability dyes to ensure specific staining of live cells.
• While the antibody is validated for surface staining, fixation and permeabilization may be considered if intracellular detection is required, though the epitope is extracellular.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for IVD raw materials, from antibody development to bulk ancillary reagents. This anti-CD58 monoclonal antibody supports research into T-cell-mediated immunity, immune synapse formation, and related diagnostic applications. Leverage our capabilities in validated antibody pairs, optimized monoclonal/polyclonal antibodies, and rare-target raw-material sourcing to streamline your IVD development.