Rabbit anti-Human CD58 (LFA-3) Monoclonal Antibody for FC - P19256

Core Product Specifications and Parameters

Biological Background: CD58 Function and Localization

• CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a surface glycoprotein that serves as a ligand for the T-lymphocyte CD2 glycoprotein.
• The CD58-CD2 interaction mediates thymocyte interactions with thymic epithelial cells, facilitating T-cell development and selection.
• It enables antigen-independent and -dependent interactions between T-lymphocytes and target cells or antigen-presenting cells, crucial for immune synapse formation.
• CD58 is involved in T-lymphocyte rosetting with erythrocytes, a classic phenomenon often used to assess T-cell function.
• In addition to its adhesive role, LFA-3/CD2 engagement may prime responses by both CD2+ and LFA-3+ cells, potentially modulating activation thresholds.
• Subcellularly, CD58 is localized at the cell membrane, consistent with its role in cell-cell communication.
• The protein undergoes alternative splicing, producing isoforms with molecular weights around 26-28 kDa.
• Post-translational modifications include disulfide bond formation and glycosylation, which are important for its structural stability and function.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (amino acids 29-215) of human CD58, designed to recognize native surface antigen.
• This conjugate is optimized for flow cytometry (FC); ensure proper compensation and use appropriate isotype controls.
• For FC, titrate the antibody to determine optimal staining concentration; typical working dilutions may be determined empirically.
• Consider using this antibody for immunofluorescence or other surface staining applications after validation.
• Since CD58 is expressed on various hematopoietic cells, include appropriate cell lineage markers for gating.
• Avoid freeze-thaw cycles; store undiluted antibody at 2–8°C protected from light.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. With this anti-CD58 antibody, researchers can accelerate immuno-oncology and immune synapse studies. CamelBio offers validated antibody pairs, monoclonal antibodies, and bulk ancillary reagents to support your diagnostic development needs.