Rabbit anti-Human CD56/NCAM1 Monoclonal Antibody for Flow Cytometry - P13591

Core Product Specifications and Parameters

Biological Background: CD56/NCAM1 Function and Localization

• CD56/NCAM1 is a cell adhesion molecule belonging to the immunoglobulin superfamily, mediating homophilic and heterophilic interactions.
• It plays a key role in neural development, including neuron-neuron adhesion, neurite fasciculation, and outgrowth.
• The protein undergoes alternative splicing, giving rise to multiple isoforms (e.g., GPI-anchored and transmembrane forms) with distinct localizations.
• Subcellularly, NCAM1 is found at the cell membrane and can also be secreted; some isoforms are tethered via a GPI anchor.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation, which are important for its adhesive functions.
• In addition to its neural functions, NCAM1 serves as a host cell receptor for rabies and Zika viruses, facilitating viral entry.

Experimental Guidance and Technical Tips

• This conjugate is suitable for direct immunofluorescence flow cytometry on human samples. Validate the optimal working concentration by titration on the relevant cell line or primary cells.
• Since the immunogen was designed using amino acids 20-413 of the extracellular region, the antibody likely recognizes surface-exposed epitopes across multiple isoforms. For intracellular or secreted forms, consider permeabilization.
• NCAM1 is expressed on neurons, natural killer cells, and some tumor types; ensure your sample system is relevant for your experimental goal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for neurobiology and infectious disease research. We also offer customized sourcing for rare targets like CD56/NCAM1, ensuring you get the right tools for your assay development.