Rabbit anti-Human CD56/NCAM1 Monoclonal Antibody for Flow Cytometry - P13591

Core Product Specifications and Parameters

Biological Background: Neural Cell Adhesion Molecule 1 Function and Localization

• NCAM1, also known as CD56, is a member of the immunoglobulin superfamily of cell adhesion molecules, encoded by the NCAM1 gene (GeneID: 4684) and existing in multiple isoforms due to alternative splicing.
• The protein mediates homophilic cell-cell adhesion, contributing to neuronal development processes such as neurite outgrowth, fasciculation, and synaptogenesis.
• NCAM1 serves as a receptor for viral entry, including rabies virus and Zika virus, playing a role in host-virus interactions.
• It is primarily localized to the cell membrane, with some isoforms secreted or tethered to the membrane via a GPI anchor; it undergoes post-translational modifications including glycosylation and phosphorylation.
• The extracellular region contains immunoglobulin-like domains and disulfide bonds, while other structural features include signal peptide, transmembrane helix, and repeat regions.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant protein corresponding to amino acids 20-413, which covers a large portion of the extracellular domain; it may recognize multiple isoforms of NCAM1.
• For flow cytometry (FC) applications, consider titrating the antibody to determine the optimal working concentration and using appropriate isotype controls.
• Since cross-reactivity is reported only for human, validate performance in your specific sample system; it may not be suitable for mouse or rat models.
• Live-cell staining could be feasible given the immunogen targets the extracellular domain, but fixation and permeabilization conditions should be optimized independently.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw-material procurement by offering a comprehensive range of antibodies, including this rabbit anti-CD56/NCAM1 monoclonal antibody, ideal for neuroscience and infectious disease research. Our portfolio covers validated antibody pairs, bulk ancillary reagents, and rare target sourcing, ensuring you have reliable access to the materials needed for diagnostic kit development and clinical research from concept to clinic.