Rabbit anti-Human CD55 Monoclonal Antibody for FC - P08174

Core Product Specifications and Parameters

Biological Background: CD55 Function and Localization

• CD55, also known as decay-accelerating factor (DAF), is a GPI-anchored membrane protein that protects cells from complement-mediated damage by inhibiting the C3 and C5 convertases. Related references: PMID:7525274 PMID:28657829
• It recognizes C4b and C3b fragments deposited on cell surfaces and interferes with the formation and stability of the C4b2a and C3bBb convertases, preventing amplification of the complement cascade. Related references: PMID:7525274 PMID:28657829
• CD55 is expressed on the plasma membranes of virtually all cell types that are in contact with plasma complement proteins, as well as on epithelial cells lining extracellular compartments; soluble variants are found in body fluids and extracellular matrix.
• Multiple isoforms exist due to alternative splicing, including both membrane-bound and secreted forms, which modulate complement activity locally and systemically.
• The protein carries the Cromer blood group antigens, and mutations in CD55 are associated with CHAPLE syndrome, a disease characterized by early-onset protein-losing enteropathy and thrombotic events.
• CD55 is rich in Sushi (CCP) domains, disulfide bonds, and glycosylation sites, contributing to its structural stability and ligand recognition.
• Beyond complement regulation, CD55 acts as a cellular receptor for several pathogens, including Coxsackievirus A21, Coxsackieviruses B1, B3, and B5, Human enteroviruses 70 and D68, and echoviruses 6, 7, 11, 12, 20, and 21.
• As a GPI-anchored protein, CD55 partitions into lipid rafts, where it cooperates with other membrane-bound complement regulators to maintain immune homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 35–353) of human CD55, suggesting that this antibody may recognize native, surface-expressed CD55—ideal for flow cytometry without permeabilization.
• is a bright, photostable fluorophore (equivalent to Alexa Fluor® 488), suitable for multicolor FC panels; consider titrating the antibody to determine the optimal dilution for your cell type.
• Validate staining specificity by pre-incubating the antibody with recombinant CD55 protein or by using a CD55-negative cell line as a control.
• For accurate measurement of surface CD55, avoid fixation/permeabilization protocols that could strip GPI-anchored proteins from the membrane.
• In combination with other complement-related markers, this antibody can help delineate subsets of immune cells or assess cytoprotective mechanisms on tumor cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with ready-to-use, validated monoclonal antibodies like this anti-CD55 conjugate, alongside complementary reagents for complement cascade analysis. Our portfolio spans bulk antibody pairs, optimized monoclonals, and ancillary raw materials to accelerate IVD kit development from concept to clinic. For rare targets or custom sourcing needs, our global supply network ensures continuity and compliance at every stage.