Rabbit anti-Human CD52 Monoclonal Antibody for Flow Cytometry - P31358

Core Product Specifications and Parameters

Biological Background: CD52 Function and Localization

• CD52, also known as CAMPATH-1 antigen, HE5, or CDW52, is a very small GPI-anchored glycoprotein with a mature peptide of only 12 amino acids after signal peptide cleavage and GPI attachment.
• As a GPI-anchored membrane protein, CD52 localizes to the cell membrane and is attached via a glycosylphosphatidylinositol (GPI) anchor, lacking a transmembrane domain.
• It is highly expressed on lymphocytes, monocytes, and other immune cells, but also found in the male reproductive tract as epididymal secretory protein E5 (HE5).
• CD52 is heavily glycosylated; the GPI anchor and N-linked glycan contribute to its carbohydrate presentation role, possibly orienting carbohydrates at the cell surface for cell-cell interactions.
• While its precise function remains elusive, CD52 may play a role in carrying and orienting carbohydrate moieties on the cell surface, with potential involvement in immune regulation.
• The CD52 antigen has a molecular weight of ~6.6 kDa (calculated from amino acid sequence) but often migrates higher due to glycosylation; this antibody targets the N-terminal region (1-61).
• CD52 is the target of the therapeutic monoclonal antibody alemtuzumab (Campath-1H), used in the treatment of chronic lymphocytic leukemia and multiple sclerosis.
• Keywords from UniProt include: 3D-structure, Cell membrane, Glycoprotein, GPI-anchor, Lipoprotein, Membrane, Reference proteome, Signal.

Experimental Guidance and Technical Tips

• This -conjugated antibody is suitable for direct flow cytometry on live human cells. Validate staining conditions (antibody concentration, incubation time, temperature) in your specific assay system.
• CD52 is a surface antigen; avoid permeabilization steps that might disrupt the GPI anchor. Use gentle cell handling and standard surface staining protocols.
• The immunogen corresponds to the N-terminal extracellular region (amino acids 1-61), ensuring recognition of the mature cell surface form.
• Strong expression is expected on peripheral blood lymphocytes and monocytes; consider using healthy donor PBMCs as a positive control.
• For quantitative flow cytometry, include appropriate isotype controls and perform titration to determine the optimal antibody concentration.
• Due to the small size and GPI linkage, CD52 may be shed from the cell surface; immediate processing of fresh samples is recommended for optimal detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, labs, and research institutes to provide a seamless pipeline of high-quality IVD raw materials. This Rabbit anti-Human CD52 monoclonal antibody is one of many validated monoclonals we offer for immuno-oncology and immunophenotyping research. Beyond individual antibodies, CamelBio specializes in sourcing rare target reagents, optimized antibody pairs, and bulk ancillary materials—supporting your development from concept to clinic.