Rabbit anti-Human CD52 Monoclonal Antibody for Flow Cytometry - P31358

Core Product Specifications and Parameters

Biological Background: CD52 Function and Localization

• CD52 (CAMPATH-1 antigen, HE5, CDW52, EDDM5) is a GPI-anchored cell-surface glycoprotein encoded by the CD52 gene in humans (GeneID: 1043).
• UniProt annotates its molecular weight at 6614 Da (~7 kDa), consistent with the product specification; a mature peptide after signal sequence cleavage.
• It localizes to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor (SUBCELLULAR LOCATION: Cell membrane; KEYWORDS: GPI-anchor).
• Functionally, CD52 is predicted to play a role in carrying and orienting carbohydrate moieties on the cell surface, though its exact biological role remains incompletely defined.
• The protein is also known as Epididymal secretory protein E5 (He5), reflecting its expression in the epididymis (Alt Names).
• Keywords from UniProt include "3D-structure", indicating available structural data; "Direct protein sequencing"; "Glycoprotein"; "Signal"; and "Reference proteome".

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 1-61 of human CD52—consider this when evaluating epitope accessibility, especially near the N-terminal signal peptide and GPI-anchor attachment site.
• This antibody is directly conjugated to , a bright and photostable fluorophore; suitable for single-color flow cytometry or multiplex panels with careful compensation.
• Since CD52 is a low-abundance GPI-anchored protein (~7 kDa), ensure adequate cell numbers, appropriate isotype controls, and optimized staining conditions for best signal-to-noise.
• For flow cytometry, use fresh or properly cryopreserved human cells; fixation/permeabilization may affect epitope recognition—validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with one-stop access to IVD raw materials, covering every stage from concept to clinic. For CD52-targeted assays, we offer validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to streamline your flow cytometry or immunophenotyping workflows. Whether you need routine catalog items or rare target sourcing, CamelBio bridges the gap between discovery and diagnostic development.