Rabbit anti-Human CD51 Monoclonal Antibody for Flow Cytometry - P06756

Core Product Specifications and Parameters

Biological Background: CD51 (Integrin alpha-V) Function and Localization

• Integrin alpha-V (CD51) heterodimerizes with various beta subunits (e.g., ITGB3, ITGB5, ITGB6, ITGB8) to form receptors that mediate cell adhesion to extracellular matrix proteins primarily through R-G-D motif recognition, with ligands including vitronectin, fibronectin, and fibrinogen.
• ITGAV:ITGB3 functions as a co-receptor for multiple growth factors and cytokines, binding FGF1 (PubMed:18441324), FGF2 (PubMed:28302677), IGF1 (PubMed:19578119), IGF2 (PubMed:28873464), IL1B (PubMed:29030430), NRG1 (PubMed:20682778), and CD40LG (PubMed:31331973), which is essential for their downstream signaling. Related references: PMID:18441324 PMID:28302677 PMID:19578119 PMID:28873464 PMID:29030430 PMID:20682778 PMID:31331973
• Alpha-V integrins are crucial for TGF-beta-1 activation; ITGAV:ITGB6 and ITGAV:ITGB8 mediate R-G-D-dependent release of TGF-beta-1 from its latency-associated peptide (LAP), a critical mechanism in fibrosis and immune regulation. Related references: PMID:15184403 PMID:22278742 PMID:28117447
• CD51 also serves as a host cell receptor for multiple viruses: ITGAV:ITGB5 and ITGAV:ITGB3 for Coxsackievirus A9 and B1, ITGAV:ITGB3 for Herpes virus 8 and West Nile virus, and ITGAV:ITGB6 for herpes simplex 1, implicating it in viral pathogenesis.
• The protein localizes primarily at the cell membrane and is enriched at focal adhesions, where it links the extracellular matrix to the actin cytoskeleton to facilitate cell migration and signal transduction.
• It also localizes to cell-cell junctions, potentially contributing to intercellular adhesion and tissue integrity.
• Post-translational processing includes cleavage into heavy and light chains, disulfide bond formation, and extensive glycosylation, which are critical for its structural integrity and functional regulation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the C-terminal region (aa 949-1048) of human CD51, suggesting the antibody recognizes an intracellular epitope. For intracellular flow cytometry, standard fixation and permeabilization protocols should be optimized to ensure epitope accessibility.
• Since this antibody is validated for flow cytometry (intracellular), it is suitable for detecting CD51 inside cells. Consider using appropriate controls, such as knockout cells or peptide-blocked antibody, to confirm specificity in your experimental system.
• Because CD51 forms heterodimers with different beta subunits, co-staining with antibodies against specific integrin beta chains may help elucidate the composition of the receptor complexes in your samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This -conjugated anti-CD51 monoclonal antibody supports flow cytometry applications in integrin alpha-V research, including cell adhesion and viral host entry studies. Beyond validated antibody reagents, CamelBio offers custom monoclonal and polyclonal antibody development, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your IVD assay development.