Rabbit anti-Human CD50(ICAM-3) Monoclonal Antibody for FC - P32942

Core Product Specifications and Parameters

Biological Background: CD50/ICAM-3 Function and Localization

• CD50 (ICAM-3, Intercellular Adhesion Molecule 3) is a heavily glycosylated type I transmembrane protein belonging to the immunoglobulin superfamily, and serves as a key cell adhesion molecule on resting leukocytes.
  Related references: PMID:1448173, PMID:1448174, PMID:23775590

• It functions as a primary ligand for the integrin LFA-1 (ITGAL/ITGB2), mediating intercellular adhesion events essential for immune surveillance, including apoptotic neutrophil phagocytosis by macrophages.
  Related references: PMID:1448173, PMID:1448174, PMID:23775590

• ICAM-3 also interacts with integrin ITGAD/ITGB2, further broadening its role in leukocyte adhesion and trafficking.
  Related references: PMID:8777714

• On dendritic cells (DCs), ICAM-3 binds to the C-type lectin CD209 (DC-SIGN) in a calcium-dependent manner, establishing initial contact with resting T cells and promoting T cell proliferation.
  Related references: PMID:10721994

• CD50 is constitutively expressed exclusively on resting leukocytes (including lymphocytes, monocytes, and neutrophils) but is absent from resting or cytokine-activated endothelial cells.

• The protein contains five immunoglobulin-like C2-type domains in its extracellular region and undergoes post-translational modifications such as N-linked glycosylation and phosphorylation, which regulate its adhesive functions.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 30-114) corresponds to the N-terminal extracellular domain, which is accessible on live cells; consider staining unfixed leukocytes for optimal recognition in flow cytometry.
• Perform a titration experiment to determine the optimal antibody concentration for your specific cell type and assay conditions, as labeling intensity can vary between leukocyte subsets.
• Include an appropriate isotype control antibody conjugated to to assess nonspecific binding and establish gating thresholds.
• For fixation and permeabilization steps, validate that the epitope recognized by this antibody is preserved, as the extracellular domain may be sensitive to certain treatments.
• This product is validated for flow cytometry; other applications may require additional optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For projects involving CD50/ICAM-3 and leukocyte adhesion, we offer comprehensive sourcing support for rare targets and custom development services. From concept to clinic, CamelBio bridges the gap between your assay requirements and high-quality raw material supply.