Rabbit anti-Human CD48 Monoclonal Antibody for Flow Cytometry - P09326

Core Product Specifications and Parameters

Biological Background: CD48 Antigen Function and Localization

• CD48 is a glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein belonging to the SLAM family (SLAMF2). It is also known as B-lymphocyte activation marker BLAST-1, BCM1 surface antigen, Leukocyte antigen MEM-102, and Signaling lymphocytic activation molecule 2.

• CD48 interacts via its N-terminal immunoglobulin domain with cell surface receptors CD244 (2B4) and CD2 to regulate immune cell function and activation. Related references: PMID:12007789 PMID:19494291 PMID:27249817

• In T-cells, CD48 associates with CD2 and facilitates the recruitment of the Src family kinase LCK and the adaptor LAT to the TCR/CD3 complex, promoting LCK phosphorylation and downstream signaling. Related references: PMID:19494291 PMID:12007789

• CD48 induces phosphorylation of the ITSMs of CD244, initiating signaling events that lead to formation of the immunological synapse and the directed release of cytolytic granules (perforin and granzymes) by T-lymphocytes and NK cells. Related references: PMID:27249817

• CD48 is widely expressed on all hematopoietic cells.

• CD48 is localized to the cell membrane, membrane rafts, and may also be secreted.

• Post-translational modifications include GPI-anchor attachment, disulfide bond formation, and glycosylation; the unprocessed precursor has a molecular weight of approximately 27.7 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region of CD48 (amino acids 27-220), likely preserving native conformation; consider using live, non-permeabilized cells for flow cytometry to maintain surface epitope integrity.
• Optimize antibody titration for your specific sample type (e.g., peripheral blood mononuclear cells, cultured lymphocytes, or NK cells) and include appropriate isotype and fluorescence-minus-one controls.
• Since CD48 is widely expressed on hematopoietic cells, validate staining specificity by comparing to known positive and negative cell populations.
• For functional studies, note that CD48 is a GPI-anchored protein; crosslinking may be required for certain activation assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to a comprehensive portfolio of IVD raw materials. This anti-CD48 monoclonal antibody supports immunological assay development and biomarker analysis, particularly in hematopoietic cell profiling. Beyond individual antibodies, CamelBio partners can source validated antibody pairs, bulk ancillary reagents, and custom rare-target raw materials for seamless diagnostic workflow integration.