Rabbit anti-Human CD47 Monoclonal Antibody for Flow Cytometry - Q08722

Core Product Specifications and Parameters

Biological Background: CD47 Function and Localization

• Leukocyte surface antigen CD47 (also known as IAP, OA3, MER6) is a widely expressed transmembrane glycoprotein belonging to the immunoglobulin superfamily, with multiple isoforms arising from alternative splicing.
• Acts as an adhesive protein that mediates cell-to-cell interactions, functioning as a receptor for thrombospondin-1 (THBS1) and modulating integrin signaling through heterotrimeric G proteins. Related references: PMID:11509594, PMID:15383453, PMID:19004835, PMID:7691831, PMID:8550562
• Plays a critical role in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation. Related references: PMID:11509594, PMID:15383453, PMID:19004835
• Serves as a receptor for SIRPA, inhibiting dendritic cell maturation and cytokine production, while interaction with SIRPG promotes cell-cell adhesion and costimulates T-cell activation. Related references: PMID:11509594, PMID:15383453
• Involved in memory formation and synaptic plasticity in the hippocampus, and positively modulates FAS-dependent apoptosis in T-cells.
• Suppresses angiogenesis and may contribute to metabolic dysregulation during normal aging. Related references: PMID:32679764
• Inhibits stem cell self-renewal in response to THBS1, likely by regulating the transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc, and KLF4.
• Broadly distributed across normal adult tissues, especially abundant in certain epithelia, the brain, and macrophages, and also found in ovarian tumors. Related references: PMID:39121194

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular domain (amino acids 19–139) of human CD47, making this antibody well-suited for detecting the native surface form of CD47.
• Directly conjugated with , a bright red fluorescent dye, this antibody is optimized for flow cytometry (FC). It can be used for single-color or multicolor panel staining without requiring a secondary reagent.
• For cell surface staining, consider using live, unfixed cells and include appropriate Fc receptor blocking to reduce nonspecific binding.
• Always run appropriate controls (e.g., isotype control and unstained cells) to set gates accurately and confirm specificity.
• CD47 expression may vary across cell types and activation states; validate staining conditions in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a comprehensive range of IVD raw materials, technical services, and consulting—from concept to clinic. This Rabbit anti-Human CD47 monoclonal antibody supports cutting-edge research in immune evasion, cancer immunotherapy, and cardiovascular studies. Beyond this reagent, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your development workflows.