Rabbit anti-Human CD47 Monoclonal Antibody for FC - Q08722

Core Product Specifications and Parameters

Biological Background: CD47 Function and Localization

• CD47, also designated as integrin-associated protein (IAP), MER6, and OA3, is a cell surface glycoprotein belonging to the immunoglobulin superfamily.
• It serves as a receptor for thrombospondin-1 (THBS1) and mediates cell-to-cell adhesion, modulating integrin signaling through heterotrimeric G proteins. Related references: PMID:11509594 PMID:15383453 PMID:19004835 PMID:7691831 PMID:8550562
• CD47 is involved in diverse physiological processes, including signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation. Related references: PMID:11509594 PMID:15383453 PMID:19004835
• It functions as a receptor for SIRPA, inhibiting dendritic cell maturation and cytokine production, and interacts with SIRPG to enhance T-cell activation. Related references: PMID:11509594 PMID:15383453
• CD47 is a transmembrane protein predominantly located at the cell membrane and is widely expressed in normal adult tissues, including epithelia and brain.
• Alternative splicing generates multiple CD47 isoforms that differ in their cytoplasmic domains, correlating with the observed molecular weights of 31–35 kDa. The protein undergoes post-translational modifications such as glycosylation and phosphorylation, and features an immunoglobulin-like domain and disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 19-139) of human CD47, suggesting that this antibody recognizes the native surface antigen, making it well-suited for live-cell flow cytometry without permeabilization.
• For flow cytometry (FC) applications, it is recommended to titrate the antibody to determine the optimal staining concentration for your cell type. A typical starting range for fluorophore-conjugated antibodies is 0.5–5 µg/mL, but empirical optimization is essential.
• Include appropriate isotype controls, Fc receptor blocking, and viability staining to ensure accurate gating of CD47-positive populations, particularly given the broad expression of CD47 across hematopoietic and non-hematopoietic cells.
• Validate antibody performance in the specific sample matrix (e.g., whole blood, tissue digests, cultured cells) and consider fixation protocols, as the dye is compatible with fixation after staining.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material sourcing partner, CamelBio provides diagnostic manufacturers and research institutions with validated antibodies, including monoclonal antibodies like this anti-CD47 clone, enzyme conjugates, and ancillary reagents essential for assay development. Beyond single products, CamelBio offers comprehensive solutions—from antibody pair screening to bulk supply and technical consultation—to accelerate the translation of CD47-targeted diagnostics and immunology research applications. Our global network enables access to rare and custom reagents, ensuring that even niche targets such as CD47 are readily available to support your pipeline from concept to clinic.