Rabbit anti-Human CD46 mAb for Flow Cytometry - P15529

Core Product Specifications and Parameters

Biological Background: CD46 Function and Localization

• CD46 is a membrane cofactor protein that protects host cells from complement attack by serving as a cofactor for factor I-mediated cleavage of C3b and C4b.
• It is expressed on all cells except erythrocytes, highlighting its fundamental role in innate immune surveillance.
• CD46 localizes to the inner acrosomal membrane of spermatozoa and participates in fertilization by facilitating sperm-oocyte fusion.
• As a T-cell costimulatory molecule, CD46 promotes the differentiation of CD4+ T-cells into regulatory Tr1 cells that secrete IL-10, thereby contributing to peripheral tolerance.
• CD46 serves as a receptor for various pathogens, including measles virus, adenoviruses (subgroup B2/Ad3), and human herpesvirus 6.
• It also mediates adhesion and invasion of pathogenic bacteria such as Neisseria and Streptococcus pyogenes. Related references: PMID:11260136 PMID:11971006 PMID:7708671
• Additional roles include a minor contribution to fiber-mediated entry of adenovirus types 55 and 14a. Related references: PMID:32581096
• Post-translational modifications encompass glycosylation and phosphorylation, and alternative splicing generates functionally diverse isoforms. Mutations are linked to atypical hemolytic uremic syndrome.

Experimental Guidance and Technical Tips

• When using this conjugate for flow cytometry, consider titrating the antibody to determine optimal staining concentration and include appropriate isotype and fluorescence-minus-one controls.
• The immunogen spans the extracellular Sushi domains (amino acids 35-328), suggesting the antibody recognizes the native CD46 ectodomain; validate reactivity in the intended sample system.
• CD46 is expressed on most nucleated cells but absent on erythrocytes, which can serve as a negative control population.
• For standard surface staining, use live, unfixed cells; intracellular detection may require fixation and permeabilization, which should be validated experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to supply high-quality IVD raw materials, including validated monoclonal antibodies like this anti-CD46 reagent for flow cytometry. Whether you need bulk quantities of catalog antibodies, customized antibody pairs, or rare-target protein reagents, we offer streamlined sourcing and technical support to accelerate your assay development. Leverage CamelBio’s supply network to secure the critical immune-relevant and infectious disease research reagents you need from concept to clinic.