Rabbit Anti-Human CD45RB Monoclonal Antibody (PE) for Flow Cytometry - P08575-9

Core Product Specifications and Parameters

Biological Background: CD45RB Function and Localization

• CD45RB is an isoform of receptor-type tyrosine-protein phosphatase C (PTPRC), encoded by the PTPRC gene.
• It is also known as leukocyte common antigen (L-CA) and is abundantly expressed on the surface of all leukocytes.
• As a receptor-type tyrosine phosphatase, it plays a critical role in the regulation of immune cell signaling by dephosphorylating phosphotyrosine residues of key signaling molecules.
• The protein localizes to the plasma membrane and has an approximate molecular weight of 136 kDa.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 26-463 of human CD45RB, corresponding to a large portion of the extracellular domain; the resulting antibody is expected to recognize the native surface receptor, making it well suited for flow cytometry applications.
• This antibody is supplied as a PE conjugate, enabling direct detection without the need for secondary staining; titrate the antibody concentration for optimal signal-to-noise ratio in your specific assay.
• Include appropriate isotype controls and single-stain compensation controls when performing multicolor flow cytometry to ensure accurate data analysis.
• Validate the antibody in your cell line or primary cell system of interest, and consider fixation and permeabilization conditions if intracellular epitopes are targeted.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers, laboratories, and research institutes, providing one-stop access to IVD raw materials. Our catalog features validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for leukocyte research. We also specialize in sourcing rare-target raw materials, with this CD45RB antibody enabling exploration of immune cell biology and related disorders.