Rabbit anti-Human CD45RA Monoclonal Antibody for Flow Cytometry - P08575-8

Core Product Specifications and Parameters

Biological Background: CD45RA Function and Localization

• CD45RA is isoform 6 of the receptor-type tyrosine-protein phosphatase C (PTPRC), a transmembrane enzyme with protein tyrosine phosphatase activity.
• It is widely recognized as the leukocyte common antigen (L-CA) and T200, reflecting its abundant expression across all nucleated hematopoietic cells.
• The PTPRC gene undergoes alternative splicing to generate multiple isoforms; CD45RA includes the A exon, defining its distinct extracellular domain.
• The predicted molecular weight of this isoform is approximately 138 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 26‑482) of CD45RA, supporting strong and specific binding in flow cytometry applications.
• As a directly conjugated antibody, no secondary staining step is required; however, optimal working concentration should be determined by titration on the target cell population.
• Include appropriate isotype controls and perform live/dead cell discrimination to ensure accurate gating when analyzing human leukocyte subsets.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to supplying diagnostic manufacturers and research laboratories with high-quality IVD raw materials, including validated antibody pairs, bulk monoclonal antibodies, and ancillary reagents. This anti-CD45RA monoclonal antibody supports precise leukocyte immunophenotyping for immunology research and clinical diagnostics. Through a comprehensive procurement network, CamelBio streamlines access to both routine and hard-to-find raw materials, accelerating your development from concept to clinic.