Rabbit anti-Human CD45RA Monoclonal Antibody for Flow Cytometry - P08575-8

Core Product Specifications and Parameters

Biological Background: CD45RA Function and Localization

• CD45RA is isoform 6 of receptor-type tyrosine-protein phosphatase C (PTPRC), encoded by the PTPRC gene (CD45).
• It is commonly referred to as leukocyte common antigen (L-CA), T200, or CD45, and belongs to the protein tyrosine phosphatase family.
• The PTPRC gene generates multiple isoforms through alternative splicing; isoform 6 (CD45RA) contains specific extracellular segments.
• The predicted molecular weight of this isoform is approximately 138 kDa.
• As a receptor-type phosphatase, CD45 localizes to the plasma membrane, where it plays a key role in signal transduction within hematopoietic cells.
• CD45RA expression is characteristic of certain leukocyte subsets, including naïve T cells, and is widely used as a phenotypic marker in immunology.

Experimental Guidance and Technical Tips

• Since the immunogen is a recombinant protein covering the extracellular domain (amino acids 26-482), the antibody is expected to recognize the native conformation of CD45RA on the cell surface.
• For flow cytometry, it is advisable to titrate the antibody to determine the optimal staining concentration for your specific cell type and instrument settings.
• Include appropriate isotype control antibodies and viability dyes to ensure accurate gating and minimize nonspecific binding.
• Validate antibody performance in the relevant sample system (e.g., human peripheral blood mononuclear cells) to confirm specificity and sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For immunophenotyping and hematopoietic research, we offer a range of anti-CD45 and other leukocyte marker antibodies to support your diagnostic assay development. From concept to clinic, our one-stop platform streamlines procurement and technical consulting for reliable supply of high-quality bioreagents.